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http://purl.uniprot.org/citations/8606183http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/8606183http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/8606183http://www.w3.org/2000/01/rdf-schema#comment"The locus glc (min 64.5), associated with the glycolate utilization trait in Escherichia coli, is known to contain glcB, encoding malate synthase G, and the gene(s) needed for glycolate oxidase activity. Subcloning, sequencing, insertion mutagenesis, and expression studies showed five additional genes: glcC and in the other direction glcD, glcE, glcF, and glcG followed by glcB. The gene glcC may encode the glc regulator protein. Consistently a chloramphenicol acetyltransferase insertion mutation abolished both glycolate oxidase and malate synthase G activities. The proteins encoded from glcD and glcE displayed similarity to several flavoenzymes, the one from glcF was found to be similar to iron-sulfur proteins, and that from glcG had no significant similarity to any group of proteins. The insertional mutation by a chloramphenicol acetyltransferase cassette in either glcD, glcE, or glcF abolished glycolate oxidase activity, indicating that presumably these proteins are subunits of this enzyme. No effect on glycolate metabolism was detected by insertional mutation in glcG. Northern (RNA) blot experiments showed constitutive expression of glcC but induced expression for the structural genes and provided no evidence for a single polycistronic transcript."xsd:string
http://purl.uniprot.org/citations/8606183http://purl.org/dc/terms/identifier"doi:10.1128/jb.178.7.2051-2059.1996"xsd:string
http://purl.uniprot.org/citations/8606183http://purl.org/dc/terms/identifier"doi:10.1128/jb.178.7.2051-2059.1996"xsd:string
http://purl.uniprot.org/citations/8606183http://purl.uniprot.org/core/author"Badia J."xsd:string
http://purl.uniprot.org/citations/8606183http://purl.uniprot.org/core/author"Badia J."xsd:string
http://purl.uniprot.org/citations/8606183http://purl.uniprot.org/core/author"Baldoma L."xsd:string
http://purl.uniprot.org/citations/8606183http://purl.uniprot.org/core/author"Baldoma L."xsd:string
http://purl.uniprot.org/citations/8606183http://purl.uniprot.org/core/author"Pellicer M.T."xsd:string
http://purl.uniprot.org/citations/8606183http://purl.uniprot.org/core/author"Pellicer M.T."xsd:string
http://purl.uniprot.org/citations/8606183http://purl.uniprot.org/core/author"Aguilar J.T."xsd:string
http://purl.uniprot.org/citations/8606183http://purl.uniprot.org/core/author"Aguilar J.T."xsd:string
http://purl.uniprot.org/citations/8606183http://purl.uniprot.org/core/date"1996"xsd:gYear
http://purl.uniprot.org/citations/8606183http://purl.uniprot.org/core/date"1996"xsd:gYear
http://purl.uniprot.org/citations/8606183http://purl.uniprot.org/core/name"J. Bacteriol."xsd:string
http://purl.uniprot.org/citations/8606183http://purl.uniprot.org/core/name"J. Bacteriol."xsd:string
http://purl.uniprot.org/citations/8606183http://purl.uniprot.org/core/pages"2051-2059"xsd:string
http://purl.uniprot.org/citations/8606183http://purl.uniprot.org/core/pages"2051-2059"xsd:string
http://purl.uniprot.org/citations/8606183http://purl.uniprot.org/core/title"glc locus of Escherichia coli: characterization of genes encoding the subunits of glycolate oxidase and the glc regulator protein."xsd:string
http://purl.uniprot.org/citations/8606183http://purl.uniprot.org/core/title"glc locus of Escherichia coli: characterization of genes encoding the subunits of glycolate oxidase and the glc regulator protein."xsd:string
http://purl.uniprot.org/citations/8606183http://purl.uniprot.org/core/volume"178"xsd:string
http://purl.uniprot.org/citations/8606183http://purl.uniprot.org/core/volume"178"xsd:string
http://purl.uniprot.org/citations/8606183http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/8606183
http://purl.uniprot.org/citations/8606183http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/8606183