| http://purl.uniprot.org/citations/8606964 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8606964 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8606964 | http://www.w3.org/2000/01/rdf-schema#comment | "Regulation of beta 1-adrenergic receptors is unusual in developing animals. For example, glucocorticoid-and thyroid hormone-responsiveness for several genes is seen in animals treated during fetal life but beta 1-responsiveness is not seen until after birth. In order to investigate this at the transcriptional level, the ovine beta 1 receptor gene was cloned from a sheep genomic library. An approach using high-stringency screening with cDNA probes and oligonucleotides from regions of human and rat genes conserved but unique to the beta 1 receptor but not to other seven transmembrane, G-protein-coupled receptors. Over 800,000 clones were screened from which 40-50 positive clones were identified by each of the probes. There was, however, only a single clone which was recognized by each of the probes. A 5-kb insert was subcloned and shown to contain sequences which hybridized to each of the probes. Using the restriction map of the rat beta 1 receptor, a 1.0-kb Pst1 internal fragment was further subcloned for sequence identification. Confirmation of this fragment as the ovine beta 1 receptor was based on homology of the beta 1 receptor from other species and tissue distribution of mRNA. Nucleotide sequence homology was 93% with the human beta 1 receptor and 84% with rat. Amino acid sequence homology was > 75% and approached 100% in the transmembrane regions. The approach described represents a practical approach to cloning and identification of hormone receptors from the highly homologous members of the seven-transmembrane, G-protein-coupled receptors."xsd:string |
| http://purl.uniprot.org/citations/8606964 | http://purl.org/dc/terms/identifier | "doi:10.1071/rd9950521"xsd:string |
| http://purl.uniprot.org/citations/8606964 | http://purl.org/dc/terms/identifier | "doi:10.1071/rd9950521"xsd:string |
| http://purl.uniprot.org/citations/8606964 | http://purl.uniprot.org/core/author | "Padbury J.F."xsd:string |
| http://purl.uniprot.org/citations/8606964 | http://purl.uniprot.org/core/author | "Padbury J.F."xsd:string |
| http://purl.uniprot.org/citations/8606964 | http://purl.uniprot.org/core/author | "Tseng Y.-T."xsd:string |
| http://purl.uniprot.org/citations/8606964 | http://purl.uniprot.org/core/author | "Tseng Y.-T."xsd:string |
| http://purl.uniprot.org/citations/8606964 | http://purl.uniprot.org/core/author | "Waschek J.A."xsd:string |
| http://purl.uniprot.org/citations/8606964 | http://purl.uniprot.org/core/author | "Waschek J.A."xsd:string |
| http://purl.uniprot.org/citations/8606964 | http://purl.uniprot.org/core/date | "1995"xsd:gYear |
| http://purl.uniprot.org/citations/8606964 | http://purl.uniprot.org/core/date | "1995"xsd:gYear |
| http://purl.uniprot.org/citations/8606964 | http://purl.uniprot.org/core/name | "Reprod. Fertil. Dev."xsd:string |
| http://purl.uniprot.org/citations/8606964 | http://purl.uniprot.org/core/name | "Reprod. Fertil. Dev."xsd:string |
| http://purl.uniprot.org/citations/8606964 | http://purl.uniprot.org/core/pages | "521-525"xsd:string |
| http://purl.uniprot.org/citations/8606964 | http://purl.uniprot.org/core/pages | "521-525"xsd:string |
| http://purl.uniprot.org/citations/8606964 | http://purl.uniprot.org/core/title | "A cloning strategy for G-protein-coupled hormone receptors: the ovine beta 1-adrenergic receptor."xsd:string |
| http://purl.uniprot.org/citations/8606964 | http://purl.uniprot.org/core/title | "A cloning strategy for G-protein-coupled hormone receptors: the ovine beta 1-adrenergic receptor."xsd:string |
| http://purl.uniprot.org/citations/8606964 | http://purl.uniprot.org/core/volume | "7"xsd:string |
| http://purl.uniprot.org/citations/8606964 | http://purl.uniprot.org/core/volume | "7"xsd:string |
| http://purl.uniprot.org/citations/8606964 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/8606964 |
| http://purl.uniprot.org/citations/8606964 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/8606964 |
| http://purl.uniprot.org/citations/8606964 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/8606964 |
| http://purl.uniprot.org/citations/8606964 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/8606964 |