RDF/XMLNTriplesTurtleShow queryShare
SubjectPredicateObject
http://purl.uniprot.org/citations/8612752http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/8612752http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/8612752http://www.w3.org/2000/01/rdf-schema#comment"A full-length genomic DNA fragment that codes for a novel EF-hand protein Dictyostelium discoideum was cloned and sequenced. The protein is composed of 168 amino acids and contains four consensus sequences that are typical for (Ca2+)-binding EF-hand domains. The protein sequence exhibits only minor similarities to other calmodulin-type proteins from Dictyostelium. The genomic DNA harbors two short introns; their positions suggest that the gene is unrelated to the EF-hand proteins from the calmodulin group. Northern blot analysis showed that the mRNA level was significantly increased during development. Polyclonal antibodies raised against the recombinant protein recognized in Western blots a protein of about 20 kDa. Like the mRNA, also the protein was more abundant in developing cells. Overlay experiments with 45Ca2+ indicated that the EF-hands in fact have (Ca2+)-binding activity. The recent description of CBP1, another calmodulin-type Dictyostelium protein that is upregulated during development [Coukell et al. (1995) FEBS Lett. 362, 342-346], suggests that D. discoideum contains a family of EF-hand proteins that have specific functions during distinct steps of development. We therefore designate the protein described in this report as CBP2."xsd:string
http://purl.uniprot.org/citations/8612752http://purl.org/dc/terms/identifier"doi:10.1016/0014-5793(96)00176-7"xsd:string
http://purl.uniprot.org/citations/8612752http://purl.org/dc/terms/identifier"doi:10.1016/0014-5793(96)00176-7"xsd:string
http://purl.uniprot.org/citations/8612752http://purl.uniprot.org/core/author"Noegel A.A."xsd:string
http://purl.uniprot.org/citations/8612752http://purl.uniprot.org/core/author"Noegel A.A."xsd:string
http://purl.uniprot.org/citations/8612752http://purl.uniprot.org/core/author"Schleicher M."xsd:string
http://purl.uniprot.org/citations/8612752http://purl.uniprot.org/core/author"Schleicher M."xsd:string
http://purl.uniprot.org/citations/8612752http://purl.uniprot.org/core/author"Andre B."xsd:string
http://purl.uniprot.org/citations/8612752http://purl.uniprot.org/core/author"Andre B."xsd:string
http://purl.uniprot.org/citations/8612752http://purl.uniprot.org/core/date"1996"xsd:gYear
http://purl.uniprot.org/citations/8612752http://purl.uniprot.org/core/date"1996"xsd:gYear
http://purl.uniprot.org/citations/8612752http://purl.uniprot.org/core/name"FEBS Lett."xsd:string
http://purl.uniprot.org/citations/8612752http://purl.uniprot.org/core/name"FEBS Lett."xsd:string
http://purl.uniprot.org/citations/8612752http://purl.uniprot.org/core/pages"198-202"xsd:string
http://purl.uniprot.org/citations/8612752http://purl.uniprot.org/core/pages"198-202"xsd:string
http://purl.uniprot.org/citations/8612752http://purl.uniprot.org/core/title"Dictyostelium discoideum contains a family of calmodulin-related EF-hand proteins that are developmentally regulated."xsd:string
http://purl.uniprot.org/citations/8612752http://purl.uniprot.org/core/title"Dictyostelium discoideum contains a family of calmodulin-related EF-hand proteins that are developmentally regulated."xsd:string
http://purl.uniprot.org/citations/8612752http://purl.uniprot.org/core/volume"382"xsd:string
http://purl.uniprot.org/citations/8612752http://purl.uniprot.org/core/volume"382"xsd:string
http://purl.uniprot.org/citations/8612752http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/8612752
http://purl.uniprot.org/citations/8612752http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/8612752
http://purl.uniprot.org/citations/8612752http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/8612752
http://purl.uniprot.org/citations/8612752http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/8612752