| http://purl.uniprot.org/citations/8617268 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8617268 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8617268 | http://www.w3.org/2000/01/rdf-schema#comment | "The gene encoding the elongation factor Ts from Thermus thermophilus was sequenced, cloned and the protein overproduced in Escherichia coli. In comparison to the EF-Ts from E. coli with 282 amino acid residues, EF-Ts from T. thermophilus is considerably shorter, differing by 86 amino acids. EF-Ts from the thermophile is stable at high temperatures, which facilitates its separation from E. coli proteins. Purified T. thermophilus EF-Ts forms a homodimer with a disulfide bridge between the two cysteine residues at position 190. The modification of Cys19O by iodoacetamide affects neither the dimerization nor the ability of EF-Ts to facilitate the nucleotide exchange of elongation factor Tu. The disulfide bridge was detected only in purified EF-TS, but not in protein extracts immediately after cell disruption. The physiological role of this disulfide bridge remains, therefore, unclear. Besides the quaternary (EF-TU . EF-Ts)2 complex, a ternary EF-TU . EF-Ts2 complex was detected by gel permeation chromatography and polyacrylamide gel electrophoresis. Trypsin cleavage after Lys48 or modification of Cys78 yield inactive EF-Ts, that does not bind to EF-Tu but is still capable of forming homodimers."xsd:string |
| http://purl.uniprot.org/citations/8617268 | http://purl.org/dc/terms/identifier | "doi:10.1111/j.1432-1033.1996.00222.x"xsd:string |
| http://purl.uniprot.org/citations/8617268 | http://purl.org/dc/terms/identifier | "doi:10.1111/j.1432-1033.1996.00222.x"xsd:string |
| http://purl.uniprot.org/citations/8617268 | http://purl.uniprot.org/core/author | "Blank J."xsd:string |
| http://purl.uniprot.org/citations/8617268 | http://purl.uniprot.org/core/author | "Blank J."xsd:string |
| http://purl.uniprot.org/citations/8617268 | http://purl.uniprot.org/core/author | "Kreutzer R."xsd:string |
| http://purl.uniprot.org/citations/8617268 | http://purl.uniprot.org/core/author | "Kreutzer R."xsd:string |
| http://purl.uniprot.org/citations/8617268 | http://purl.uniprot.org/core/author | "Nock S."xsd:string |
| http://purl.uniprot.org/citations/8617268 | http://purl.uniprot.org/core/author | "Nock S."xsd:string |
| http://purl.uniprot.org/citations/8617268 | http://purl.uniprot.org/core/author | "Sprinzl M."xsd:string |
| http://purl.uniprot.org/citations/8617268 | http://purl.uniprot.org/core/author | "Sprinzl M."xsd:string |
| http://purl.uniprot.org/citations/8617268 | http://purl.uniprot.org/core/date | "1996"xsd:gYear |
| http://purl.uniprot.org/citations/8617268 | http://purl.uniprot.org/core/date | "1996"xsd:gYear |
| http://purl.uniprot.org/citations/8617268 | http://purl.uniprot.org/core/name | "Eur. J. Biochem."xsd:string |
| http://purl.uniprot.org/citations/8617268 | http://purl.uniprot.org/core/name | "Eur. J. Biochem."xsd:string |
| http://purl.uniprot.org/citations/8617268 | http://purl.uniprot.org/core/pages | "222-227"xsd:string |
| http://purl.uniprot.org/citations/8617268 | http://purl.uniprot.org/core/pages | "222-227"xsd:string |
| http://purl.uniprot.org/citations/8617268 | http://purl.uniprot.org/core/title | "Elongation factor Ts from Thermus thermophilus -- overproduction in Escherichia coli, quaternary structure and interaction with elongation factor Tu."xsd:string |
| http://purl.uniprot.org/citations/8617268 | http://purl.uniprot.org/core/title | "Elongation factor Ts from Thermus thermophilus -- overproduction in Escherichia coli, quaternary structure and interaction with elongation factor Tu."xsd:string |
| http://purl.uniprot.org/citations/8617268 | http://purl.uniprot.org/core/volume | "236"xsd:string |
| http://purl.uniprot.org/citations/8617268 | http://purl.uniprot.org/core/volume | "236"xsd:string |
| http://purl.uniprot.org/citations/8617268 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/8617268 |
| http://purl.uniprot.org/citations/8617268 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/8617268 |