| http://purl.uniprot.org/citations/8619474 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8619474 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8619474 | http://www.w3.org/2000/01/rdf-schema#comment | "The efficiency of shotgun DNA sequencing depends to a great extent on the quality of the random-subclone libraries used. We here describe a novel "double adaptor" strategy for efficient construction of high-quality shotgun libraries. In this method, randomly sheared and end-repaired fragments are ligated to oligonucleotide adaptors creating 12-base overhangs. Nonphosphorylated oligonucleotides are used, which prevents formation of adaptor dimers and ensures efficient ligation of insert to adaptor. The vector is prepared from a modified M13 vector, by KpnI/PstI digestion followed by ligation to oligonucleotides with ends complementary to the overhangs created in the digest. These adaptors create 5'-overhangs complementary to those on the inserts. Following annealing of insert to vector, the DNA is directly used for transformation without a ligation step. This protocol is robust and shows three-to fivefold higher yield of clones compared to previous protocols. No chimeric clones can be detected and the background of clones without an insert is <1%. The procedure is rapid and shows potential for automation."xsd:string |
| http://purl.uniprot.org/citations/8619474 | http://purl.org/dc/terms/identifier | "doi:10.1006/abio.1996.0138"xsd:string |
| http://purl.uniprot.org/citations/8619474 | http://purl.org/dc/terms/identifier | "doi:10.1006/abio.1996.0138"xsd:string |
| http://purl.uniprot.org/citations/8619474 | http://purl.uniprot.org/core/author | "Andersson B."xsd:string |
| http://purl.uniprot.org/citations/8619474 | http://purl.uniprot.org/core/author | "Andersson B."xsd:string |
| http://purl.uniprot.org/citations/8619474 | http://purl.uniprot.org/core/author | "Gibbs R.A."xsd:string |
| http://purl.uniprot.org/citations/8619474 | http://purl.uniprot.org/core/author | "Gibbs R.A."xsd:string |
| http://purl.uniprot.org/citations/8619474 | http://purl.uniprot.org/core/author | "Liu W."xsd:string |
| http://purl.uniprot.org/citations/8619474 | http://purl.uniprot.org/core/author | "Liu W."xsd:string |
| http://purl.uniprot.org/citations/8619474 | http://purl.uniprot.org/core/author | "Ricafrente J.Y."xsd:string |
| http://purl.uniprot.org/citations/8619474 | http://purl.uniprot.org/core/author | "Ricafrente J.Y."xsd:string |
| http://purl.uniprot.org/citations/8619474 | http://purl.uniprot.org/core/author | "Wentland M.A."xsd:string |
| http://purl.uniprot.org/citations/8619474 | http://purl.uniprot.org/core/author | "Wentland M.A."xsd:string |
| http://purl.uniprot.org/citations/8619474 | http://purl.uniprot.org/core/date | "1996"xsd:gYear |
| http://purl.uniprot.org/citations/8619474 | http://purl.uniprot.org/core/date | "1996"xsd:gYear |
| http://purl.uniprot.org/citations/8619474 | http://purl.uniprot.org/core/name | "Anal. Biochem."xsd:string |
| http://purl.uniprot.org/citations/8619474 | http://purl.uniprot.org/core/name | "Anal Biochem"xsd:string |
| http://purl.uniprot.org/citations/8619474 | http://purl.uniprot.org/core/pages | "107-113"xsd:string |
| http://purl.uniprot.org/citations/8619474 | http://purl.uniprot.org/core/pages | "107-113"xsd:string |
| http://purl.uniprot.org/citations/8619474 | http://purl.uniprot.org/core/title | "A "double adaptor" method for improved shotgun library construction."xsd:string |
| http://purl.uniprot.org/citations/8619474 | http://purl.uniprot.org/core/title | "A "double adaptor" method for improved shotgun library construction."xsd:string |
| http://purl.uniprot.org/citations/8619474 | http://purl.uniprot.org/core/volume | "236"xsd:string |
| http://purl.uniprot.org/citations/8619474 | http://purl.uniprot.org/core/volume | "236"xsd:string |