| http://purl.uniprot.org/citations/8621737 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8621737 | http://www.w3.org/2000/01/rdf-schema#comment | "The synapsins are a family of neuron-specific phosphoproteins that selectively bind to small synaptic vesicles in the presynaptic nerve terminal. The human synapsin I gene was functionally analyzed to identify control elements directing the neuron-specific expression of synapsin I. By directly measuring the mRNA transcripts of a reporter gene, we demonstrate that the proximal region of the synapsin I promoter is sufficient for directing neuron-specific gene expression. This proximal region is highly conserved between mouse and human. Deletion of a putative binding site for the zinc finger protein, neuron-restrictive silencer factor/RE-1 silencing transcription factor (NRSF/REST), abolished neuron-specific expression of the reporter gene almost entirely, allowing constitutively acting elements of the promoter to direct expression in a non-tissue-specific manner. These constitutive transcriptional elements are present as a bipartite enhancer, consisting of the region upstream (nucleotides -422 to -235) and downstream (nucleotides -199 to -143) of the putative NRSF/REST-binding site. The latter contains a motif identical to the cAMP response element. Both regions are not active or are only weakly active in promoting transcription on their own and show no tissue-specific preference. From these data we conclude that neuron-specific expression of synapsin I is accomplished by a negative regulatory mechanism via the NRSF/REST binding motif."xsd:string |
| http://purl.uniprot.org/citations/8621737 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.271.6.3317"xsd:string |
| http://purl.uniprot.org/citations/8621737 | http://purl.uniprot.org/core/author | "Thiel G."xsd:string |
| http://purl.uniprot.org/citations/8621737 | http://purl.uniprot.org/core/author | "Schoch S."xsd:string |
| http://purl.uniprot.org/citations/8621737 | http://purl.uniprot.org/core/author | "Cibelli G."xsd:string |
| http://purl.uniprot.org/citations/8621737 | http://purl.uniprot.org/core/date | "1996"xsd:gYear |
| http://purl.uniprot.org/citations/8621737 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/8621737 | http://purl.uniprot.org/core/pages | "3317-3323"xsd:string |
| http://purl.uniprot.org/citations/8621737 | http://purl.uniprot.org/core/title | "Neuron-specific gene expression of synapsin I. Major role of a negative regulatory mechanism."xsd:string |
| http://purl.uniprot.org/citations/8621737 | http://purl.uniprot.org/core/volume | "271"xsd:string |
| http://purl.uniprot.org/citations/8621737 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/8621737 |
| http://purl.uniprot.org/citations/8621737 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/8621737 |
| http://purl.uniprot.org/uniprot/#_O88935-mappedCitation-8621737 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/8621737 |
| http://purl.uniprot.org/uniprot/O88935 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/8621737 |