| http://purl.uniprot.org/citations/8622664 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8622664 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8622664 | http://www.w3.org/2000/01/rdf-schema#comment | "In the DNA binding domain of microphthalmia-associated transcription factor (MITF), four mutations are reported: mi, Mi wh, mi ew, and mi or. MITFs encoded by the mi, Mi wh, mi ew, and Mi or mutant alleles (mi-MITF, Mi wh-MITF, Mi ew-MITF, and Mi or-MITF, respectively) interfered with the DNA binding of wild-type MITF, TFE3, and another basic helix-loop-helix leucine zipper protein in vitro. Polyclonal antibody against MITF was produced and used for investigating the subcellular localization of mutant MITFs. Immunocytochemistry and immunoblotting revealed that more than 99% of wild-type MITF and Mi wh-MITF located in nuclei of transfected NIH 3T3 and 293T cells. In contrast, mi-MITF predominantly located in the cytoplasm of cells transfected with the corresponding plasmid. When the immunoglobulin G (IgG)-conjugated peptides representing a part of the DNA binding domain containing mi and Mi wh mutations were microinjected into the cytoplasm of NRK49F cells, wild-type peptide and Mi wh-type peptide-IgG conjugate localized in nuclei but mi-type peptide-IgG conjugate was detectable only in the cytoplasm. It was also demonstrated that the nuclear translocation potential of Mi or-MITF was normal but that Mi ew-MITF was impaired as well as mi-MITF. In cotransfection assay, a strong dominant negative effect of Mi wh-MITF against wild-type MITF-dependent transactivation system on tyrosinase promoter was observed, but mi-MITF had a small effect. However, by the conjugation of simian virus 40 large-T-antigen-derived nuclear localization signal to mi-MITF, the dominant negative effect was enhanced. Furthermore, we demonstrated that the interaction between wild-type MITF and mi-MITF occurred in the cytoplasm and that mi-MITF had an inhibitory effect on nuclear localization potential of wild-type MITF."xsd:string |
| http://purl.uniprot.org/citations/8622664 | http://purl.org/dc/terms/identifier | "doi:10.1128/mcb.16.3.1203"xsd:string |
| http://purl.uniprot.org/citations/8622664 | http://purl.org/dc/terms/identifier | "doi:10.1128/mcb.16.3.1203"xsd:string |
| http://purl.uniprot.org/citations/8622664 | http://purl.uniprot.org/core/author | "Kuroki T."xsd:string |
| http://purl.uniprot.org/citations/8622664 | http://purl.uniprot.org/core/author | "Kuroki T."xsd:string |
| http://purl.uniprot.org/citations/8622664 | http://purl.uniprot.org/core/author | "Kitamura Y."xsd:string |
| http://purl.uniprot.org/citations/8622664 | http://purl.uniprot.org/core/author | "Kitamura Y."xsd:string |
| http://purl.uniprot.org/citations/8622664 | http://purl.uniprot.org/core/author | "Nomura S."xsd:string |
| http://purl.uniprot.org/citations/8622664 | http://purl.uniprot.org/core/author | "Nomura S."xsd:string |
| http://purl.uniprot.org/citations/8622664 | http://purl.uniprot.org/core/author | "Arnheiter H."xsd:string |
| http://purl.uniprot.org/citations/8622664 | http://purl.uniprot.org/core/author | "Arnheiter H."xsd:string |
| http://purl.uniprot.org/citations/8622664 | http://purl.uniprot.org/core/author | "Morii E."xsd:string |
| http://purl.uniprot.org/citations/8622664 | http://purl.uniprot.org/core/author | "Morii E."xsd:string |
| http://purl.uniprot.org/citations/8622664 | http://purl.uniprot.org/core/author | "Munakata H."xsd:string |
| http://purl.uniprot.org/citations/8622664 | http://purl.uniprot.org/core/author | "Munakata H."xsd:string |
| http://purl.uniprot.org/citations/8622664 | http://purl.uniprot.org/core/author | "Takebayashi K."xsd:string |
| http://purl.uniprot.org/citations/8622664 | http://purl.uniprot.org/core/author | "Takebayashi K."xsd:string |
| http://purl.uniprot.org/citations/8622664 | http://purl.uniprot.org/core/author | "Chida K."xsd:string |
| http://purl.uniprot.org/citations/8622664 | http://purl.uniprot.org/core/author | "Chida K."xsd:string |
| http://purl.uniprot.org/citations/8622664 | http://purl.uniprot.org/core/author | "Tsukamoto I."xsd:string |
| http://purl.uniprot.org/citations/8622664 | http://purl.uniprot.org/core/author | "Tsukamoto I."xsd:string |
| http://purl.uniprot.org/citations/8622664 | http://purl.uniprot.org/core/date | "1996"xsd:gYear |
| http://purl.uniprot.org/citations/8622664 | http://purl.uniprot.org/core/date | "1996"xsd:gYear |