| http://purl.uniprot.org/citations/8626065 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8626065 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8626065 | http://www.w3.org/2000/01/rdf-schema#comment | "We have cloned and sequenced a 3574-bp Bacillus subtilis (Bs) DNA fragment located between the nrdA and citB genes at about 169 degrees on the chromosome. An Escherichia coli strain, LBG1605, carrying a mutated ptsH gene (encoding HPr (His-containing protein) of the bacterial phosphotransferase system (PTS)) and complemented for PTS activity with the ptsH of Staphylococcus carnosus, exhibited reduced mannitol fermentation activity when transformed with a plasmid bearing this 3574-bp Bs fragment. This fragment contained an incomplete and two complete open reading frames (ORFs). The product of the first complete ORF, a protein composed of 235 amino acids (aa) (25038 Da), was found to be responsible for the observed reduced mannitol fermentation. The 3' part of this 705-bp second ORF and the 428-bp incomplete first ORF encode aa sequences exhibiting almost 40% sequence identify. However, the function of these two proteins remains unknown. The third ORF, the 1893-bp prkA gene, encodes a protein (PrkA) of 72889 Da. PrkA possesses the A-motif of nucleotide-binding proteins and exhibits distant homology to eukaryotic protein kinases. Several of the essential aa in the loops known to form the active site of cyclic adenosine 3',5'-monophosphate (cAMP)-dependent protein kinase appeared to be conserved in PrkA. After expression of prkA and purification of PrkA, we could demonstrate that PrkA can indeed phosphorylate a Bs 60-kDa protein at a Ser residue."xsd:string |
| http://purl.uniprot.org/citations/8626065 | http://purl.org/dc/terms/identifier | "doi:10.1016/0378-1119(95)00758-x"xsd:string |
| http://purl.uniprot.org/citations/8626065 | http://purl.org/dc/terms/identifier | "doi:10.1016/0378-1119(95)00758-x"xsd:string |
| http://purl.uniprot.org/citations/8626065 | http://purl.uniprot.org/core/author | "Deutscher J."xsd:string |
| http://purl.uniprot.org/citations/8626065 | http://purl.uniprot.org/core/author | "Deutscher J."xsd:string |
| http://purl.uniprot.org/citations/8626065 | http://purl.uniprot.org/core/author | "Fischer C."xsd:string |
| http://purl.uniprot.org/citations/8626065 | http://purl.uniprot.org/core/author | "Fischer C."xsd:string |
| http://purl.uniprot.org/citations/8626065 | http://purl.uniprot.org/core/author | "Geourjon C."xsd:string |
| http://purl.uniprot.org/citations/8626065 | http://purl.uniprot.org/core/author | "Geourjon C."xsd:string |
| http://purl.uniprot.org/citations/8626065 | http://purl.uniprot.org/core/author | "Bourson C."xsd:string |
| http://purl.uniprot.org/citations/8626065 | http://purl.uniprot.org/core/author | "Bourson C."xsd:string |
| http://purl.uniprot.org/citations/8626065 | http://purl.uniprot.org/core/date | "1996"xsd:gYear |
| http://purl.uniprot.org/citations/8626065 | http://purl.uniprot.org/core/date | "1996"xsd:gYear |
| http://purl.uniprot.org/citations/8626065 | http://purl.uniprot.org/core/name | "Gene"xsd:string |
| http://purl.uniprot.org/citations/8626065 | http://purl.uniprot.org/core/name | "Gene"xsd:string |
| http://purl.uniprot.org/citations/8626065 | http://purl.uniprot.org/core/pages | "55-60"xsd:string |
| http://purl.uniprot.org/citations/8626065 | http://purl.uniprot.org/core/pages | "55-60"xsd:string |
| http://purl.uniprot.org/citations/8626065 | http://purl.uniprot.org/core/title | "Cloning and characterization of the Bacillus subtilis prkA gene encoding a novel serine protein kinase."xsd:string |
| http://purl.uniprot.org/citations/8626065 | http://purl.uniprot.org/core/title | "Cloning and characterization of the Bacillus subtilis prkA gene encoding a novel serine protein kinase."xsd:string |
| http://purl.uniprot.org/citations/8626065 | http://purl.uniprot.org/core/volume | "168"xsd:string |
| http://purl.uniprot.org/citations/8626065 | http://purl.uniprot.org/core/volume | "168"xsd:string |
| http://purl.uniprot.org/citations/8626065 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/8626065 |
| http://purl.uniprot.org/citations/8626065 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/8626065 |