Results

RDF/XMLNTriplesTurtleShow queryShare ▶
SubjectPredicateObject
http://purl.uniprot.org/citations/8626090http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/8626090http://www.w3.org/2000/01/rdf-schema#comment"

Background

From previous experiments it is known that the murine dominant cataract mutants carrying the gene Cat2 have a decreased content of gamma-crystallin-specific transcripts in the juvenile lens, when the cataract is completely expressed. Moreover, the mutant locus has been mapped recently to chromosome 1, closely linked to the gamma E-crystallin gene (map distance 0.3 +/-0.3 cM). In the present paper we describe the phenotypic changes and the gamma-crystallin expression in embryonic lenses of the Cat2nop mutants as an example for the Cat2 allelic series.

Methods

The technique of in situ hybridization was applied using a probe from the murine gamma D-crystallin gene, and, for control, from the murine alpha A-crystallin gene. Simultaneously, a series of lens sections was examined histologically.

Results

The presence of gamma-crystallin mRNA was demonstrated from embryonic day 13.5 (E13.5) onward, but in the mutants to a lower extent than in the wild-type lenses. However, the first morphological abnormality in the mutant lenses was observed as swelling of lens fibers at day E15.5. Progressive degeneration of the lens core followed, leading to a cataracta immatura.

Conclusion

The reduced level of gamma-crystallin transcripts is the first alteration observable during the embryonic development of the Cat2 mutant lenses: it precedes the morphological changes. This result represents an additional line of argument that the gamma-crystallin genes may be the target of the mutation in the Cat2 mice."xsd:string
http://purl.uniprot.org/citations/8626090http://purl.org/dc/terms/identifier"doi:10.1007/bf00184093"xsd:string
http://purl.uniprot.org/citations/8626090http://purl.uniprot.org/core/author"Graw J."xsd:string
http://purl.uniprot.org/citations/8626090http://purl.uniprot.org/core/author"Santhiya S.T."xsd:string
http://purl.uniprot.org/citations/8626090http://purl.uniprot.org/core/author"Loster J."xsd:string
http://purl.uniprot.org/citations/8626090http://purl.uniprot.org/core/author"Abd-alla S.M."xsd:string
http://purl.uniprot.org/citations/8626090http://purl.uniprot.org/core/date"1995"xsd:gYear
http://purl.uniprot.org/citations/8626090http://purl.uniprot.org/core/name"Graefes Arch Clin Exp Ophthalmol"xsd:string
http://purl.uniprot.org/citations/8626090http://purl.uniprot.org/core/pages"795-800"xsd:string
http://purl.uniprot.org/citations/8626090http://purl.uniprot.org/core/title"Reduced levels of gamma-crystallin transcripts during embryonic development of murine Cat2nop mutant lenses."xsd:string
http://purl.uniprot.org/citations/8626090http://purl.uniprot.org/core/volume"233"xsd:string
http://purl.uniprot.org/citations/8626090http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/8626090
http://purl.uniprot.org/citations/8626090http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/8626090
http://purl.uniprot.org/uniprot/P04344#attribution-94B866311ADA1F9314D35405440AD5AChttp://purl.uniprot.org/core/sourcehttp://purl.uniprot.org/citations/8626090
http://purl.uniprot.org/uniprot/#_A0A494B9Q6-mappedCitation-8626090http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/8626090
http://purl.uniprot.org/uniprot/#_F7CUK9-mappedCitation-8626090http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/8626090
http://purl.uniprot.org/uniprot/#_Q546L9-mappedCitation-8626090http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/8626090
http://purl.uniprot.org/uniprot/#_P04342-mappedCitation-8626090http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/8626090
http://purl.uniprot.org/uniprot/#_P04344-mappedCitation-8626090http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/8626090
http://purl.uniprot.org/uniprot/#_P04345-mappedCitation-8626090http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/8626090
http://purl.uniprot.org/uniprot/#_P24622-mappedCitation-8626090http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/8626090
http://purl.uniprot.org/uniprot/#_Q569M7-mappedCitation-8626090http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/8626090
http://purl.uniprot.org/uniprot/Q569M7http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/8626090
http://purl.uniprot.org/uniprot/P04344http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/8626090
▶