http://purl.uniprot.org/citations/8631322 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/8631322 | http://www.w3.org/2000/01/rdf-schema#comment | "We have purified a DNA helicase from Drosophila embryos by following unwinding activity during the purification of the cellular single-stranded DNA-binding protein dRP-A. This DNA helicase unwinds DNA 5' to 3', has a salt-tolerant activity, and has a preference for purine triphosphates as cofactors for the unwinding reaction. The purified enzyme consists of a single polypeptide of 120 kDa, which cosediments with the helicase activity. Sedimentation analysis suggests that this polypeptide exists as a monomer under high and low salt conditions. Dhel II is able to unwind long stretches of DNA, but with decreased efficiency. Addition of Escherichia coli-like single-stranded DNA-binding proteins stimulates the unwinding activity at least 10-fold on substrates greater than 200 nucleotides. In particular, the mitochondrial single-stranded DNA-binding protein isolated from Drosophila embryos is able to stimulate unwinding by dhel II. These properties show that the helicase described is different from another Drosophila helicase dhel I; it has thus has been classified as dhel II."xsd:string |
http://purl.uniprot.org/citations/8631322 | http://purl.org/dc/terms/identifier | "doi:10.1111/j.1432-1033.1996.00152.x"xsd:string |
http://purl.uniprot.org/citations/8631322 | http://purl.uniprot.org/core/author | "Cotterill S."xsd:string |
http://purl.uniprot.org/citations/8631322 | http://purl.uniprot.org/core/author | "Thommes P."xsd:string |
http://purl.uniprot.org/citations/8631322 | http://purl.uniprot.org/core/author | "Marton R.F."xsd:string |
http://purl.uniprot.org/citations/8631322 | http://purl.uniprot.org/core/date | "1996"xsd:gYear |
http://purl.uniprot.org/citations/8631322 | http://purl.uniprot.org/core/name | "Eur J Biochem"xsd:string |
http://purl.uniprot.org/citations/8631322 | http://purl.uniprot.org/core/pages | "152-158"xsd:string |
http://purl.uniprot.org/citations/8631322 | http://purl.uniprot.org/core/title | "Isolation and characterisation of dhel II, a DNA helicase from Drosophila melanogaster embryos stimulated by Escherichia coli-type single-stranded-DNA-binding proteins."xsd:string |
http://purl.uniprot.org/citations/8631322 | http://purl.uniprot.org/core/volume | "235"xsd:string |
http://purl.uniprot.org/citations/8631322 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/8631322 |
http://purl.uniprot.org/citations/8631322 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/8631322 |
http://purl.uniprot.org/uniprot/#_P54622-mappedCitation-8631322 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/8631322 |
http://purl.uniprot.org/uniprot/P54622 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/8631322 |