| http://purl.uniprot.org/citations/8631707 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8631707 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8631707 | http://www.w3.org/2000/01/rdf-schema#comment | "A flagellin gene from the fish pathogen Vibrio anguillarum was cloned, sequenced, and mutagenized. The DNA sequence suggests that the flaA gene encodes a 40.1-kDa protein and is a single transcriptional unit. A polar mutation and four in-frame deletion mutations (180 bp deleted from the 5' end of the gene, 153 bp deleted from the 3' end of the gene, a double deletion of both the 180- and 153-bp deletions, and 942 bp deleted from the entire gene) were made. Compared with the wild type, all mutants were partially motile, and a shortening of the flagellum was seen by electron microscopy. Wild-type phenotypes were regained when the mutations were transcomplemented with the flaA gene. Protein analysis indicated that the flaA gene corresponds to a 40-kDa protein and that the flagellum consists of three additional flagellin proteins with molecular masses of 41, 42, and 45 kDa. N-terminal sequence analysis confirmed that the additional proteins were flagellins with N termini that are 82 to 88% identical to the N terminus of FlaA. Virulence studies showed that the N terminal deletion, the double deletion, and the 942-bp deletion increased the 50% lethal dose between 70- and 700-fold via immersion infection, whereas infection via intraperitoneal injection showed no loss in virulence. In contrast, the polar mutant and the carboxy-terminal deletion mutant showed approximately a 10(4)-fold increase in the 50% lethal dose by both immersion and intraperitoneal infection. In summary, FlaA is needed for crossing the fish integument and may play a role in virulence after invasion of the host."xsd:string |
| http://purl.uniprot.org/citations/8631707 | http://purl.org/dc/terms/identifier | "doi:10.1128/jb.178.5.1310-1319.1996"xsd:string |
| http://purl.uniprot.org/citations/8631707 | http://purl.org/dc/terms/identifier | "doi:10.1128/jb.178.5.1310-1319.1996"xsd:string |
| http://purl.uniprot.org/citations/8631707 | http://purl.uniprot.org/core/author | "Milton D.L."xsd:string |
| http://purl.uniprot.org/citations/8631707 | http://purl.uniprot.org/core/author | "Milton D.L."xsd:string |
| http://purl.uniprot.org/citations/8631707 | http://purl.uniprot.org/core/author | "O'Toole R."xsd:string |
| http://purl.uniprot.org/citations/8631707 | http://purl.uniprot.org/core/author | "O'Toole R."xsd:string |
| http://purl.uniprot.org/citations/8631707 | http://purl.uniprot.org/core/author | "Wolf-Watz H."xsd:string |
| http://purl.uniprot.org/citations/8631707 | http://purl.uniprot.org/core/author | "Wolf-Watz H."xsd:string |
| http://purl.uniprot.org/citations/8631707 | http://purl.uniprot.org/core/author | "Hoerstedt P."xsd:string |
| http://purl.uniprot.org/citations/8631707 | http://purl.uniprot.org/core/author | "Hoerstedt P."xsd:string |
| http://purl.uniprot.org/citations/8631707 | http://purl.uniprot.org/core/date | "1996"xsd:gYear |
| http://purl.uniprot.org/citations/8631707 | http://purl.uniprot.org/core/date | "1996"xsd:gYear |
| http://purl.uniprot.org/citations/8631707 | http://purl.uniprot.org/core/name | "J. Bacteriol."xsd:string |
| http://purl.uniprot.org/citations/8631707 | http://purl.uniprot.org/core/name | "J. Bacteriol."xsd:string |
| http://purl.uniprot.org/citations/8631707 | http://purl.uniprot.org/core/pages | "1310-1319"xsd:string |
| http://purl.uniprot.org/citations/8631707 | http://purl.uniprot.org/core/pages | "1310-1319"xsd:string |
| http://purl.uniprot.org/citations/8631707 | http://purl.uniprot.org/core/title | "Flagellin A is essential for the virulence of Vibrio anguillarum."xsd:string |
| http://purl.uniprot.org/citations/8631707 | http://purl.uniprot.org/core/title | "Flagellin A is essential for the virulence of Vibrio anguillarum."xsd:string |
| http://purl.uniprot.org/citations/8631707 | http://purl.uniprot.org/core/volume | "178"xsd:string |
| http://purl.uniprot.org/citations/8631707 | http://purl.uniprot.org/core/volume | "178"xsd:string |
| http://purl.uniprot.org/citations/8631707 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/8631707 |
| http://purl.uniprot.org/citations/8631707 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/8631707 |