| http://purl.uniprot.org/citations/8631956 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8631956 | http://www.w3.org/2000/01/rdf-schema#comment | "The Caenorhabditis elegans cell death gene, ced-3, encodes one of the two proteins required for apoptosis in this organism. The primary sequence similarities between Ced-3 and the mammalian interleukin-1beta converting enzyme (ICE) suggest that these two proteins may have functionally similar active sites and that Ced-3 may function as a cysteine protease. Here we report that in vitro transcribed and translated Ced-3 protein (p56) underwent rapid processing to smaller fragments. Replacement of the predicted active site cysteine of Ced-3 with serine (C364S) prevented the generation of smaller proteolytic fragments, suggesting that the processing might be an autocatalytic process. Peptide aldehydes with aspartic acid at the P1 position blocked Ced-3 autocatalysis. Furthermore, the protease inhibition profile of Ced-3 was similar to the profile reported for ICE. These functional data demonstrate that Ced-3 is an Asp-dependent cysteine protease with substrate specificity similar to that of ICE. Aurintricarboxylic acid, an inhibitor of apoptosis in mammalian cells, blocked Ced-3 autocatalytic activity, suggesting that an aurintricarboxylic acid-sensitive Ced-3/ICE-related protease might be involved in the apoptosis pathway(s) in mammalian cells."xsd:string |
| http://purl.uniprot.org/citations/8631956 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.271.7.3517"xsd:string |
| http://purl.uniprot.org/citations/8631956 | http://purl.uniprot.org/core/author | "Mankovich J.A."xsd:string |
| http://purl.uniprot.org/citations/8631956 | http://purl.uniprot.org/core/author | "Ghayur T."xsd:string |
| http://purl.uniprot.org/citations/8631956 | http://purl.uniprot.org/core/author | "Hugunin M."xsd:string |
| http://purl.uniprot.org/citations/8631956 | http://purl.uniprot.org/core/author | "Quintal L.J."xsd:string |
| http://purl.uniprot.org/citations/8631956 | http://purl.uniprot.org/core/date | "1996"xsd:gYear |
| http://purl.uniprot.org/citations/8631956 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/8631956 | http://purl.uniprot.org/core/pages | "3517-3522"xsd:string |
| http://purl.uniprot.org/citations/8631956 | http://purl.uniprot.org/core/title | "Protease activity of in vitro transcribed and translated Caenorhabditis elegans cell death gene (ced-3) product."xsd:string |
| http://purl.uniprot.org/citations/8631956 | http://purl.uniprot.org/core/volume | "271"xsd:string |
| http://purl.uniprot.org/citations/8631956 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/8631956 |
| http://purl.uniprot.org/citations/8631956 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/8631956 |
| http://purl.uniprot.org/uniprot/P42573#attribution-7372B5A42123A812447EDF21422B2DFF | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/8631956 |
| http://purl.uniprot.org/uniprot/D3YT61#attribution-7372B5A42123A812447EDF21422B2DFF | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/8631956 |
| http://purl.uniprot.org/uniprot/#_D3YT61-mappedCitation-8631956 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/8631956 |
| http://purl.uniprot.org/uniprot/#_P42573-mappedCitation-8631956 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/8631956 |
| http://purl.uniprot.org/uniprot/P42573 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/8631956 |
| http://purl.uniprot.org/uniprot/D3YT61 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/8631956 |