| http://purl.uniprot.org/citations/8639509 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8639509 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8639509 | http://www.w3.org/2000/01/rdf-schema#comment | "alphaB-Crystallin, originally described as a structural lens protein, is now known to be a member of the small heat shock protein family and is expressed in a number of nonlens tissues. This highly conserved 20 kDa protein aggregates with homologous proteins, including alphaA-crystallin and the small heat shock protein HSP28, to form large heteromeric complexes. Recently, Roquemore et al. (1992) have established that both phosphorylated and unphosphorylated forms of lens alphaB-crystallin are modified with O-linked N-acetylglucosamine, a dynamic posttranslational modification abundant on nuclear and cytoplasmic proteins. In this paper, we have identified the major site of O-GlcNAcylation on lens alphaB as Thr 170. We have further shown that this modification is not restricted to lens alphaB-crystallin but occurs on alphaB isolated from rat heart tissue and human astroglioma cells. Two-dimensional electrophoresis of rat heart alphaB-crystallin revealed two O-GlcNAcylated forms with mobilities corresponding to the unphosphorylated form (alphaB2) and an unidentified, slightly more acidic form. Phosphorylated alphaB-crystallin (alphaB1) was not detected in the rat heart preparation. The major O-GlcNAcylation site on alphaB-crystallins from rat heart also appears to be at Thr 170. Metabolic pulse-chase labeling studies of U373-MG astroglioma cells indicated that turnover of the carbohydrate on alphaB-crystallin is not static but proceeds many-fold more rapidly than turnover of the protein backbone itself, consistent with a regulatory role for O-GlcNAc on this small heat shock protein."xsd:string |
| http://purl.uniprot.org/citations/8639509 | http://purl.org/dc/terms/identifier | "doi:10.1021/bi951918j"xsd:string |
| http://purl.uniprot.org/citations/8639509 | http://purl.org/dc/terms/identifier | "doi:10.1021/bi951918j"xsd:string |
| http://purl.uniprot.org/citations/8639509 | http://purl.uniprot.org/core/author | "Hart G.W."xsd:string |
| http://purl.uniprot.org/citations/8639509 | http://purl.uniprot.org/core/author | "Hart G.W."xsd:string |
| http://purl.uniprot.org/citations/8639509 | http://purl.uniprot.org/core/author | "Cotter R.J."xsd:string |
| http://purl.uniprot.org/citations/8639509 | http://purl.uniprot.org/core/author | "Cotter R.J."xsd:string |
| http://purl.uniprot.org/citations/8639509 | http://purl.uniprot.org/core/author | "Chevrier M.R."xsd:string |
| http://purl.uniprot.org/citations/8639509 | http://purl.uniprot.org/core/author | "Chevrier M.R."xsd:string |
| http://purl.uniprot.org/citations/8639509 | http://purl.uniprot.org/core/author | "Roquemore E.P."xsd:string |
| http://purl.uniprot.org/citations/8639509 | http://purl.uniprot.org/core/author | "Roquemore E.P."xsd:string |
| http://purl.uniprot.org/citations/8639509 | http://purl.uniprot.org/core/date | "1996"xsd:gYear |
| http://purl.uniprot.org/citations/8639509 | http://purl.uniprot.org/core/date | "1996"xsd:gYear |
| http://purl.uniprot.org/citations/8639509 | http://purl.uniprot.org/core/name | "Biochemistry"xsd:string |
| http://purl.uniprot.org/citations/8639509 | http://purl.uniprot.org/core/name | "Biochemistry"xsd:string |
| http://purl.uniprot.org/citations/8639509 | http://purl.uniprot.org/core/pages | "3578-3586"xsd:string |
| http://purl.uniprot.org/citations/8639509 | http://purl.uniprot.org/core/pages | "3578-3586"xsd:string |
| http://purl.uniprot.org/citations/8639509 | http://purl.uniprot.org/core/title | "Dynamic O-GlcNAcylation of the small heat shock protein alpha B-crystallin."xsd:string |
| http://purl.uniprot.org/citations/8639509 | http://purl.uniprot.org/core/title | "Dynamic O-GlcNAcylation of the small heat shock protein alpha B-crystallin."xsd:string |
| http://purl.uniprot.org/citations/8639509 | http://purl.uniprot.org/core/volume | "35"xsd:string |
| http://purl.uniprot.org/citations/8639509 | http://purl.uniprot.org/core/volume | "35"xsd:string |
| http://purl.uniprot.org/citations/8639509 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/8639509 |
| http://purl.uniprot.org/citations/8639509 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/8639509 |