| http://purl.uniprot.org/citations/8639592 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8639592 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8639592 | http://www.w3.org/2000/01/rdf-schema#comment | "Human tissue factor pathway inhibitor is a protease inhibitor with three tandem Kunitz-type inhibitory domains. The recombinant protein (r-hTFPI) was produced using Chinese hamster ovary cells, and its polypeptide and carbohydrate chain structures were analyzed. The complete amino acid sequence, composed of 276 residues, was determined using a protein sequencer after protease digestion and it was identical to that predicted from the cDNA sequence. Among three potential N-glycosylation sites, both Asn117 and Asn167 were fully N-glycosylated but Asn228 was not. Thr175 was also fully O-glycosylated, but Ser174 was partially O-glycosylated. Carbohydrate composition and mass spectrometric analyses of the undecapeptide OG-11 (residues Leu 170approximately Leu180) showed that two O-linked carbohydrate chains consisted of a type-1 core structure (Gal-GalNAc-Ser/Thr) with 0-3 mol of N-acetylneuraminic acid(s). The N-linked carbohydrate chains were analyzed by two-dimensional carbohydrate mapping combined with sequential glycosidase digestion, after the reducing-ends of carbohydrate residues were tagged with 2-aminopyridine and non-reducing-end sialic acids were removed with sialidase. All the N-linked structures in r-hTFPI were complex-type carbohydrate chains with one fucose residue attached to the reducing-end GlcNAc and consisted of bi-, tri-, and tetraantennary carbohydrate chains in the ratio 1.9:1.3:1.0. Fucosylated tri- and tetraantennary carbohydrate chains with one or two N-acetyllactosaminyl repeats were also found (30% of carbohydrate chains determined). Thus, the region between Kunitz domains 2 and 3 encoded by exon 7 was highly glycosylated by two O-linked carbohydrate chains at Ser174 and Thr175 and one N-linked carbohydrate chain at Asn167. These results indicated that the region is occupied by a cluster of three bulky and acidic carbohydrate chains."xsd:string |
| http://purl.uniprot.org/citations/8639592 | http://purl.org/dc/terms/identifier | "doi:10.1021/bi9524880"xsd:string |
| http://purl.uniprot.org/citations/8639592 | http://purl.org/dc/terms/identifier | "doi:10.1021/bi9524880"xsd:string |
| http://purl.uniprot.org/citations/8639592 | http://purl.uniprot.org/core/author | "Kato H."xsd:string |
| http://purl.uniprot.org/citations/8639592 | http://purl.uniprot.org/core/author | "Kato H."xsd:string |
| http://purl.uniprot.org/citations/8639592 | http://purl.uniprot.org/core/author | "Miyata T."xsd:string |
| http://purl.uniprot.org/citations/8639592 | http://purl.uniprot.org/core/author | "Miyata T."xsd:string |
| http://purl.uniprot.org/citations/8639592 | http://purl.uniprot.org/core/author | "Tsunasawa S."xsd:string |
| http://purl.uniprot.org/citations/8639592 | http://purl.uniprot.org/core/author | "Tsunasawa S."xsd:string |
| http://purl.uniprot.org/citations/8639592 | http://purl.uniprot.org/core/author | "Miyagi M."xsd:string |
| http://purl.uniprot.org/citations/8639592 | http://purl.uniprot.org/core/author | "Miyagi M."xsd:string |
| http://purl.uniprot.org/citations/8639592 | http://purl.uniprot.org/core/author | "Nakahara Y."xsd:string |
| http://purl.uniprot.org/citations/8639592 | http://purl.uniprot.org/core/author | "Nakahara Y."xsd:string |
| http://purl.uniprot.org/citations/8639592 | http://purl.uniprot.org/core/author | "Funatsu A."xsd:string |
| http://purl.uniprot.org/citations/8639592 | http://purl.uniprot.org/core/author | "Funatsu A."xsd:string |
| http://purl.uniprot.org/citations/8639592 | http://purl.uniprot.org/core/author | "Hamuro T."xsd:string |
| http://purl.uniprot.org/citations/8639592 | http://purl.uniprot.org/core/author | "Hamuro T."xsd:string |
| http://purl.uniprot.org/citations/8639592 | http://purl.uniprot.org/core/date | "1996"xsd:gYear |
| http://purl.uniprot.org/citations/8639592 | http://purl.uniprot.org/core/date | "1996"xsd:gYear |
| http://purl.uniprot.org/citations/8639592 | http://purl.uniprot.org/core/name | "Biochemistry"xsd:string |
| http://purl.uniprot.org/citations/8639592 | http://purl.uniprot.org/core/name | "Biochemistry"xsd:string |
| http://purl.uniprot.org/citations/8639592 | http://purl.uniprot.org/core/pages | "6450-6459"xsd:string |
| http://purl.uniprot.org/citations/8639592 | http://purl.uniprot.org/core/pages | "6450-6459"xsd:string |