| http://purl.uniprot.org/citations/8639748 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8639748 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8639748 | http://www.w3.org/2000/01/rdf-schema#comment | "Two cDNA clones, LJAS1 and LJAS2, encoding different asparagine synthetases (AS) have been identified and sequenced and their expression in Lotus japonicus characterised. Analysis of predicted amino acid sequences indicted a high level of identity with other plant AS sequences. No other AS genes were detected in the L. japonicus genome. LJAS1 gene expression was found to be root-enhanced and lower levels of transcript were also identified in photosynthetic tissues. In contrast, LJAS2 gene expression was root-specific. These patterns of AS gene expression are different from those seen in pea. AS gene expression was monitored throughout a 16 h light/8 h dark day, under nitrate-sufficient conditions. Neither transcript showed the dark-enhanced accumulation patterns previously reported for other plant AS genes. To evaluate AS activity, the molecular dynamics of asparagine synthesis were examined in vivo using 15N-ammonium labelling. A constant rate of asparagine synthesis in the roots was observed. Asparagine was the most predominant amino-component of the xylem sap and became labelled at a slightly slower rate than the asparagine in the roots, indicating that most root asparagine was located in a cytoplasmic 'transport' pool rather than in a vacuolar 'storage' pool. The steady-state mRNA levels and the 15N-labelling data suggest that light regulation of AS gene expression is not a factor controlling N-assimilation in L. japonicus roots during stable growth in N-sufficient conditions."xsd:string |
| http://purl.uniprot.org/citations/8639748 | http://purl.org/dc/terms/identifier | "doi:10.1007/bf00020801"xsd:string |
| http://purl.uniprot.org/citations/8639748 | http://purl.org/dc/terms/identifier | "doi:10.1007/bf00020801"xsd:string |
| http://purl.uniprot.org/citations/8639748 | http://purl.uniprot.org/core/author | "Clarkson D.T."xsd:string |
| http://purl.uniprot.org/citations/8639748 | http://purl.uniprot.org/core/author | "Clarkson D.T."xsd:string |
| http://purl.uniprot.org/citations/8639748 | http://purl.uniprot.org/core/author | "Massoneau A."xsd:string |
| http://purl.uniprot.org/citations/8639748 | http://purl.uniprot.org/core/author | "Massoneau A."xsd:string |
| http://purl.uniprot.org/citations/8639748 | http://purl.uniprot.org/core/author | "Prosser I.M."xsd:string |
| http://purl.uniprot.org/citations/8639748 | http://purl.uniprot.org/core/author | "Prosser I.M."xsd:string |
| http://purl.uniprot.org/citations/8639748 | http://purl.uniprot.org/core/author | "Smyth A.J."xsd:string |
| http://purl.uniprot.org/citations/8639748 | http://purl.uniprot.org/core/author | "Smyth A.J."xsd:string |
| http://purl.uniprot.org/citations/8639748 | http://purl.uniprot.org/core/author | "Waterhouse R.N."xsd:string |
| http://purl.uniprot.org/citations/8639748 | http://purl.uniprot.org/core/author | "Waterhouse R.N."xsd:string |
| http://purl.uniprot.org/citations/8639748 | http://purl.uniprot.org/core/date | "1996"xsd:gYear |
| http://purl.uniprot.org/citations/8639748 | http://purl.uniprot.org/core/date | "1996"xsd:gYear |
| http://purl.uniprot.org/citations/8639748 | http://purl.uniprot.org/core/name | "Plant Mol. Biol."xsd:string |
| http://purl.uniprot.org/citations/8639748 | http://purl.uniprot.org/core/name | "Plant Mol. Biol."xsd:string |
| http://purl.uniprot.org/citations/8639748 | http://purl.uniprot.org/core/pages | "883-897"xsd:string |
| http://purl.uniprot.org/citations/8639748 | http://purl.uniprot.org/core/pages | "883-897"xsd:string |
| http://purl.uniprot.org/citations/8639748 | http://purl.uniprot.org/core/title | "Molecular cloning and characterisation of asparagine synthetase from Lotus japonicus: dynamics of asparagine synthesis in N-sufficient conditions."xsd:string |
| http://purl.uniprot.org/citations/8639748 | http://purl.uniprot.org/core/title | "Molecular cloning and characterisation of asparagine synthetase from Lotus japonicus: dynamics of asparagine synthesis in N-sufficient conditions."xsd:string |
| http://purl.uniprot.org/citations/8639748 | http://purl.uniprot.org/core/volume | "30"xsd:string |
| http://purl.uniprot.org/citations/8639748 | http://purl.uniprot.org/core/volume | "30"xsd:string |