| http://purl.uniprot.org/citations/8643382 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8643382 | http://www.w3.org/2000/01/rdf-schema#comment | "Spinach chloroplast petD gene encodes subunit IV of the cytochrome b6/f complex. Like many chloroplast mRNAs, the spinach petD mRNA contains a 3'UTR stem-loop structure that determines correct 3' processing of the pre-mRNA and stability of the mature mRNA. RNA-protein interactions with this structure may be involved in the regulation of petD mRNA processing and/or stability. In this report, a spinach chloroplast 41 kD protein has been identified as a component of the petD mRNA 3'stem-loop:protein complex. The 41 kD protein has been purified and four internal peptide sequences were obtained. Based on the peptide sequences, a 585 bp fragment was amplified from a spinach cDNA library by PCR. And, a 1404 bp cDNA clone was then isolated by using the initial PCR fragment as probe. Nucleic acid sequence analysis of the cDNA clone identified an ORF encoding a predicted 36 kD mature protein. An apparent N-terminal transit peptide in the coding region and a 3' poly(A) tail exist in the cDNA clone indicated that this chloroplast protein as nuclear encoded. The mature protein was expressed in E. coli, and the expressed product with a molecular mass of 41 kD was purified. A gel mobility shift assay demonstrated that the expressed 41 kD protein interacts with the petD mRNA 3' UTR to form an RNA-protein complex."xsd:string |
| http://purl.uniprot.org/citations/8643382 | http://purl.uniprot.org/core/author | "Yang J."xsd:string |
| http://purl.uniprot.org/citations/8643382 | http://purl.uniprot.org/core/author | "Stern D.B."xsd:string |
| http://purl.uniprot.org/citations/8643382 | http://purl.uniprot.org/core/author | "Monde R.A."xsd:string |
| http://purl.uniprot.org/citations/8643382 | http://purl.uniprot.org/core/author | "Usack L."xsd:string |
| http://purl.uniprot.org/citations/8643382 | http://purl.uniprot.org/core/date | "1995"xsd:gYear |
| http://purl.uniprot.org/citations/8643382 | http://purl.uniprot.org/core/name | "Nucleic Acids Symp Ser"xsd:string |
| http://purl.uniprot.org/citations/8643382 | http://purl.uniprot.org/core/pages | "237-239"xsd:string |
| http://purl.uniprot.org/citations/8643382 | http://purl.uniprot.org/core/title | "The 41 kDa protein component of the spinach chloroplast petD mRNA 3' stem-loop:protein complex is a nuclear encoded chloroplast RNA-binding protein."xsd:string |
| http://purl.uniprot.org/citations/8643382 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/8643382 |
| http://purl.uniprot.org/citations/8643382 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/8643382 |
| http://purl.uniprot.org/uniprot/#_P00166-mappedCitation-8643382 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/8643382 |
| http://purl.uniprot.org/uniprot/P00166 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/8643382 |