| http://purl.uniprot.org/citations/8643509 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8643509 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8643509 | http://www.w3.org/2000/01/rdf-schema#comment | "The yeast two-hybrid system was used to isolate a clone from a 17-day-old mouse embryo cDNA library that codes for a novel 812-aa long protein fragment, glucocorticoid receptor-interacting protein 1 (GRIP1), that can interact with the hormone binding domain (HBD) of the glucocorticoid receptor. In the yeast two-hybrid system and in vitro, GRIP1 interacted with the HBDs of the glucocorticoid, estrogen, and androgen receptors in a hormone-regulated manner. When fused to the DNA binding domain of a heterologous protein, the GRIP1 fragment activated a reporter gene containing a suitable enhancer site in yeast cells and in mammalian cells, indicating that GRIP1 contains a transcriptional activation domain. Overexpression of the GRIP1 fragment in mammalian cells interfered with hormone-regulated expression of mouse mammary tumor virus-chloramphenicol acetyltransferase gene and constitutive expression of cytomegalovirus-beta-galactosidase reporter gene, but not constitutive expression from a tRNA gene promoter. This selective squelching activity suggests that GRIM can interact with an essential component of the RNA polymerase II transcription machinery. Finally, while a steroid receptor HBD fused with a GAL4 DNA binding domain did not, by itself, activate transcription of a reporter gene in yeast, coexpression of this fusion protein with GRIP1 strongly activated the reporter gene. Thus, in yeast, GRIP1 can serve as a coactivator, potentiating the transactivation functions in steroid receptor HBDs, possibly by acting as a bridge between HBDs of the receptors and the basal transcription machinery."xsd:string |
| http://purl.uniprot.org/citations/8643509 | http://purl.org/dc/terms/identifier | "doi:10.1073/pnas.93.10.4948"xsd:string |
| http://purl.uniprot.org/citations/8643509 | http://purl.org/dc/terms/identifier | "doi:10.1073/pnas.93.10.4948"xsd:string |
| http://purl.uniprot.org/citations/8643509 | http://purl.uniprot.org/core/author | "Hong H."xsd:string |
| http://purl.uniprot.org/citations/8643509 | http://purl.uniprot.org/core/author | "Hong H."xsd:string |
| http://purl.uniprot.org/citations/8643509 | http://purl.uniprot.org/core/author | "Stallcup M.R."xsd:string |
| http://purl.uniprot.org/citations/8643509 | http://purl.uniprot.org/core/author | "Stallcup M.R."xsd:string |
| http://purl.uniprot.org/citations/8643509 | http://purl.uniprot.org/core/author | "Johnson D.L."xsd:string |
| http://purl.uniprot.org/citations/8643509 | http://purl.uniprot.org/core/author | "Johnson D.L."xsd:string |
| http://purl.uniprot.org/citations/8643509 | http://purl.uniprot.org/core/author | "Trivedi A."xsd:string |
| http://purl.uniprot.org/citations/8643509 | http://purl.uniprot.org/core/author | "Trivedi A."xsd:string |
| http://purl.uniprot.org/citations/8643509 | http://purl.uniprot.org/core/author | "Kohli K."xsd:string |
| http://purl.uniprot.org/citations/8643509 | http://purl.uniprot.org/core/author | "Kohli K."xsd:string |
| http://purl.uniprot.org/citations/8643509 | http://purl.uniprot.org/core/date | "1996"xsd:gYear |
| http://purl.uniprot.org/citations/8643509 | http://purl.uniprot.org/core/date | "1996"xsd:gYear |
| http://purl.uniprot.org/citations/8643509 | http://purl.uniprot.org/core/name | "Proc. Natl. Acad. Sci. U.S.A."xsd:string |
| http://purl.uniprot.org/citations/8643509 | http://purl.uniprot.org/core/name | "Proc. Natl. Acad. Sci. U.S.A."xsd:string |
| http://purl.uniprot.org/citations/8643509 | http://purl.uniprot.org/core/pages | "4948-4952"xsd:string |
| http://purl.uniprot.org/citations/8643509 | http://purl.uniprot.org/core/pages | "4948-4952"xsd:string |
| http://purl.uniprot.org/citations/8643509 | http://purl.uniprot.org/core/title | "GRIP1, a novel mouse protein that serves as a transcriptional coactivator in yeast for the hormone binding domains of steroid receptors."xsd:string |
| http://purl.uniprot.org/citations/8643509 | http://purl.uniprot.org/core/title | "GRIP1, a novel mouse protein that serves as a transcriptional coactivator in yeast for the hormone binding domains of steroid receptors."xsd:string |
| http://purl.uniprot.org/citations/8643509 | http://purl.uniprot.org/core/volume | "93"xsd:string |
| http://purl.uniprot.org/citations/8643509 | http://purl.uniprot.org/core/volume | "93"xsd:string |