| http://purl.uniprot.org/citations/8663016 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8663016 | http://www.w3.org/2000/01/rdf-schema#comment | "Caveolae are microdomains of the plasma membrane that have been implicated in signal transduction. Caveolin, a 21-24-kDa integral membrane protein, is a principal component of the caveolae membrane. Recently, we and others have identified a family of caveolin-related proteins; caveolin has been retermed caveolin-1. Caveolin-3 is most closely related to caveolin-1, but caveolin-3 mRNA is expressed only in muscle tissue types. Here, we examine (i) the expression of caveolin-3 protein in muscle tissue types and (ii) its localization within skeletal muscle fibers by immunofluorescence microscopy and subcellular fractionation. For this purpose, we generated a novel monoclonal antibody (mAb) probe that recognizes the unique N-terminal region of caveolin-3, but not other members of the caveolin gene family. A survey of tissues and muscle cell types by Western blot analysis reveals that the caveolin-3 protein is selectively expressed only in heart and skeletal muscle tissues, cardiac myocytes, and smooth muscle cells. Immunolocalization of caveolin-3 in skeletal muscle fibers demonstrates that caveolin-3 is localized to the sarcolemma (muscle cell plasma membrane) and coincides with the distribution of another muscle-specific plasma membrane marker protein, dystrophin. In addition, caveolin-3 protein expression is dramatically induced during the differentiation of C2C12 skeletal myoblasts in culture. Using differentiated C2C12 skeletal myoblasts as a model system, we observe that caveolin-3 co-fractionates with cytoplasmic signaling molecules (G-proteins and Src-like kinases) and members of the dystrophin complex (dystrophin, alpha-sarcoglycan, and beta-dystroglycan), but is clearly separated from the bulk of cellular proteins. Caveolin-3 co-immunoprecipitates with antibodies directed against dystrophin, suggesting that they are physically associated as a discrete complex. These results are consistent with previous immunoelectron microscopic studies demonstrating that dystrophin is localized to plasma membrane caveolae in smooth muscle cells."xsd:string |
| http://purl.uniprot.org/citations/8663016 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.271.25.15160"xsd:string |
| http://purl.uniprot.org/citations/8663016 | http://purl.uniprot.org/core/author | "Chu C."xsd:string |
| http://purl.uniprot.org/citations/8663016 | http://purl.uniprot.org/core/author | "Li S."xsd:string |
| http://purl.uniprot.org/citations/8663016 | http://purl.uniprot.org/core/author | "Okamoto T."xsd:string |
| http://purl.uniprot.org/citations/8663016 | http://purl.uniprot.org/core/author | "Tang Z."xsd:string |
| http://purl.uniprot.org/citations/8663016 | http://purl.uniprot.org/core/author | "Scherer P.E."xsd:string |
| http://purl.uniprot.org/citations/8663016 | http://purl.uniprot.org/core/author | "Lisanti M.P."xsd:string |
| http://purl.uniprot.org/citations/8663016 | http://purl.uniprot.org/core/author | "Song K.S."xsd:string |
| http://purl.uniprot.org/citations/8663016 | http://purl.uniprot.org/core/author | "Kohtz D.S."xsd:string |
| http://purl.uniprot.org/citations/8663016 | http://purl.uniprot.org/core/author | "Chafel M."xsd:string |
| http://purl.uniprot.org/citations/8663016 | http://purl.uniprot.org/core/date | "1996"xsd:gYear |
| http://purl.uniprot.org/citations/8663016 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/8663016 | http://purl.uniprot.org/core/pages | "15160-15165"xsd:string |
| http://purl.uniprot.org/citations/8663016 | http://purl.uniprot.org/core/title | "Expression of caveolin-3 in skeletal, cardiac, and smooth muscle cells. Caveolin-3 is a component of the sarcolemma and co-fractionates with dystrophin and dystrophin-associated glycoproteins."xsd:string |
| http://purl.uniprot.org/citations/8663016 | http://purl.uniprot.org/core/volume | "271"xsd:string |
| http://purl.uniprot.org/citations/8663016 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/8663016 |
| http://purl.uniprot.org/citations/8663016 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/8663016 |
| http://purl.uniprot.org/uniprot/P11531#attribution-5B938A8E3CE5F848C3792241DDA78188 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/8663016 |
| http://purl.uniprot.org/uniprot/#_A0A0A6YW41-mappedCitation-8663016 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/8663016 |
| http://purl.uniprot.org/uniprot/#_A0A0A6YXB6-mappedCitation-8663016 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/8663016 |
| http://purl.uniprot.org/uniprot/#_A0A023ZT56-mappedCitation-8663016 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/8663016 |
| http://purl.uniprot.org/uniprot/#_A0A023ZTV5-mappedCitation-8663016 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/8663016 |
| http://purl.uniprot.org/uniprot/#_D3Z0J2-mappedCitation-8663016 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/8663016 |