| http://purl.uniprot.org/citations/8663316 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8663316 | http://www.w3.org/2000/01/rdf-schema#comment | "The adult neuromuscular junction displays an accumulation of both the acetylcholine receptor (AChR) protein in the subneural domain of the post-synaptic membrane and the mRNAs coding for all its subunits at the level of the subjunctional "fundamental nuclei." In the course of end plate development, the epsilon-subunit, at variance with other subunits, becomes exclusively expressed at the level of the fundamental nuclei, yet at a rather late stage (around birth). To analyze the promoter region of the epsilon-subunit gene which directs its specific expression at the synapse, we used a quantitative transient in vivo expression assay in intact muscle tissue using constructs of the epsilon-subunit promoter placed upstream of the beta-galactosidase reporter gene. One crucial element for synapse-specific expression was detected between the -11 and -6 positions. Disruption of this element, either by a scanning mutation or single base mutations, greatly diminishes, or even completely inhibits, preferential expression of the transgene at the end plate. Gel shift experiments reveal the presence of a complex in nuclear muscle extracts that bind the core sequence of this element. The identification of such a site opens the possibility to identify regulatory factors responsible for compartmentalized expression at the neuromuscular junction."xsd:string |
| http://purl.uniprot.org/citations/8663316 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.271.29.17433"xsd:string |
| http://purl.uniprot.org/citations/8663316 | http://purl.uniprot.org/core/author | "Schaeffer L."xsd:string |
| http://purl.uniprot.org/citations/8663316 | http://purl.uniprot.org/core/author | "Changeux J.P."xsd:string |
| http://purl.uniprot.org/citations/8663316 | http://purl.uniprot.org/core/author | "Duclert A."xsd:string |
| http://purl.uniprot.org/citations/8663316 | http://purl.uniprot.org/core/author | "Savatier N."xsd:string |
| http://purl.uniprot.org/citations/8663316 | http://purl.uniprot.org/core/date | "1996"xsd:gYear |
| http://purl.uniprot.org/citations/8663316 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/8663316 | http://purl.uniprot.org/core/pages | "17433-17438"xsd:string |
| http://purl.uniprot.org/citations/8663316 | http://purl.uniprot.org/core/title | "Identification of an element crucial for the sub-synaptic expression of the acetylcholine receptor epsilon-subunit gene."xsd:string |
| http://purl.uniprot.org/citations/8663316 | http://purl.uniprot.org/core/volume | "271"xsd:string |
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