| http://purl.uniprot.org/citations/8665951 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8665951 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8665951 | http://www.w3.org/2000/01/rdf-schema#comment | "Semenogelin I and semenogelin II constitute the major gel-forming proteins in human semen. The gel proteins were rapidly solubilized and separated from spermatozoa in ejaculates collected at pH 9.7 in buffer containing 4 mol/l urea and dithiothreitol. This protected the semenogelins from proteolytic degradation by prostate-specific antigen, and allowed their isolation by affinity chromatography on heparin-Sepharose. Semenogelins I and II were almost selectively retained and eluted partially separated in 0.25 mol/l NaCl. Further purification was achieved by chromatography on Superose. Approximately 10-20 mg semenogelin I and 2-5 mg semenogelin II were recovered from each sample with a purity exceeding 95% as judged by SDS/PAGE. The molecular mass of semenogelin I (49 958 Da) and the major form of semenogelin II (63 539 Da) measured by mass spectrometry was consistent with the reported cDNA data. The occurrence of a second, larger form of semenogelin II was due to asparagine-linked glycosylation. The amino-termini of the purified proteins were blocked, but digestion with pyroglutamate amino-peptidase enabled the identification of amino-terminal sequences consistent with the reported cDNA data. The amino acid compositions of the purified proteins were also consistent with those derived from cDNA data. The absorption coefficients (280 nm, 1%, 1 cm) for semenogelins I and II were 5.5 and 5.4, respectively, and the isoelectric point was above pH 9.5 for both proteins."xsd:string |
| http://purl.uniprot.org/citations/8665951 | http://purl.org/dc/terms/identifier | "doi:10.1111/j.1432-1033.1996.0048q.x"xsd:string |
| http://purl.uniprot.org/citations/8665951 | http://purl.org/dc/terms/identifier | "doi:10.1111/j.1432-1033.1996.0048q.x"xsd:string |
| http://purl.uniprot.org/citations/8665951 | http://purl.uniprot.org/core/author | "Hellman J."xsd:string |
| http://purl.uniprot.org/citations/8665951 | http://purl.uniprot.org/core/author | "Hellman J."xsd:string |
| http://purl.uniprot.org/citations/8665951 | http://purl.uniprot.org/core/author | "Lilja H."xsd:string |
| http://purl.uniprot.org/citations/8665951 | http://purl.uniprot.org/core/author | "Lilja H."xsd:string |
| http://purl.uniprot.org/citations/8665951 | http://purl.uniprot.org/core/author | "Malm J."xsd:string |
| http://purl.uniprot.org/citations/8665951 | http://purl.uniprot.org/core/author | "Malm J."xsd:string |
| http://purl.uniprot.org/citations/8665951 | http://purl.uniprot.org/core/author | "Laurell C.B."xsd:string |
| http://purl.uniprot.org/citations/8665951 | http://purl.uniprot.org/core/author | "Laurell C.B."xsd:string |
| http://purl.uniprot.org/citations/8665951 | http://purl.uniprot.org/core/author | "Magnusson H."xsd:string |
| http://purl.uniprot.org/citations/8665951 | http://purl.uniprot.org/core/author | "Magnusson H."xsd:string |
| http://purl.uniprot.org/citations/8665951 | http://purl.uniprot.org/core/date | "1996"xsd:gYear |
| http://purl.uniprot.org/citations/8665951 | http://purl.uniprot.org/core/date | "1996"xsd:gYear |
| http://purl.uniprot.org/citations/8665951 | http://purl.uniprot.org/core/name | "Eur. J. Biochem."xsd:string |
| http://purl.uniprot.org/citations/8665951 | http://purl.uniprot.org/core/name | "Eur. J. Biochem."xsd:string |
| http://purl.uniprot.org/citations/8665951 | http://purl.uniprot.org/core/pages | "48-53"xsd:string |
| http://purl.uniprot.org/citations/8665951 | http://purl.uniprot.org/core/pages | "48-53"xsd:string |
| http://purl.uniprot.org/citations/8665951 | http://purl.uniprot.org/core/title | "Isolation and characterization of the major gel proteins in human semen, semenogelin I and semenogelin II."xsd:string |
| http://purl.uniprot.org/citations/8665951 | http://purl.uniprot.org/core/title | "Isolation and characterization of the major gel proteins in human semen, semenogelin I and semenogelin II."xsd:string |
| http://purl.uniprot.org/citations/8665951 | http://purl.uniprot.org/core/volume | "238"xsd:string |
| http://purl.uniprot.org/citations/8665951 | http://purl.uniprot.org/core/volume | "238"xsd:string |