| http://purl.uniprot.org/citations/8679110 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8679110 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8679110 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Citation |
| http://purl.uniprot.org/citations/8679110 | http://www.w3.org/2000/01/rdf-schema#comment | "An inducible esterase has been isolated from a liquid culture of Aspergillus niger grown on sugar-beet pulp. The enzyme was active on methyl esters of cinnamic acids, caffeic > p-coumaric > ferulic, and is therefore termed a cinnamoyl esterase. The enzyme was not active on methyl sinapinate, a good substrate for ferulic acid esterase III, which was purified previously from A. niger [Faulds and Williamson (1994) Microbiology 140, 779-787]. With methyl caffeate as substrate the enzyme had temperature and pH optima of 50 degrees C and 6.0 respectively, and a specific activity of 96.9 units per mg of protein. The purified protein (native molecular mass 145 000 Da) gave a single heavily stained band on SDS/PAGE, suggesting the protein was a dimer, and seemed to be heavily glycosylated. Isoelectric focusing gave a single band corresponding to a pl of 4.80. The pure enzyme was free of other carbohydrase activities. The activity of the pure enzyme was inhibited by more than 99% after treatment with the serine-specific protease inhibitor aminoethylbenzenesulphonylfluoride (1 mM) for 12 h. The enzyme was capable of releasing ferulic acid from sugar beet pulp."xsd:string |
| http://purl.uniprot.org/citations/8679110 | http://purl.uniprot.org/core/author | "Williamson G."xsd:string |
| http://purl.uniprot.org/citations/8679110 | http://purl.uniprot.org/core/author | "Williamson G."xsd:string |
| http://purl.uniprot.org/citations/8679110 | http://purl.uniprot.org/core/author | "Kroon P.A."xsd:string |
| http://purl.uniprot.org/citations/8679110 | http://purl.uniprot.org/core/author | "Kroon P.A."xsd:string |
| http://purl.uniprot.org/citations/8679110 | http://purl.uniprot.org/core/author | "Faulds C.B."xsd:string |
| http://purl.uniprot.org/citations/8679110 | http://purl.uniprot.org/core/author | "Faulds C.B."xsd:string |
| http://purl.uniprot.org/citations/8679110 | http://purl.uniprot.org/core/date | "1996"xsd:gYear |
| http://purl.uniprot.org/citations/8679110 | http://purl.uniprot.org/core/date | "1996"xsd:gYear |
| http://purl.uniprot.org/citations/8679110 | http://purl.uniprot.org/core/name | "Biotechnol. Appl. Biochem."xsd:string |
| http://purl.uniprot.org/citations/8679110 | http://purl.uniprot.org/core/name | "Biotechnol. Appl. Biochem."xsd:string |
| http://purl.uniprot.org/citations/8679110 | http://purl.uniprot.org/core/pages | "255-262"xsd:string |
| http://purl.uniprot.org/citations/8679110 | http://purl.uniprot.org/core/pages | "255-262"xsd:string |
| http://purl.uniprot.org/citations/8679110 | http://purl.uniprot.org/core/title | "Purification and characterization of a novel esterase induced by growth of Aspergillus niger on sugar-beet pulp."xsd:string |
| http://purl.uniprot.org/citations/8679110 | http://purl.uniprot.org/core/title | "Purification and characterization of a novel esterase induced by growth of Aspergillus niger on sugar-beet pulp."xsd:string |
| http://purl.uniprot.org/citations/8679110 | http://purl.uniprot.org/core/volume | "23"xsd:string |
| http://purl.uniprot.org/citations/8679110 | http://purl.uniprot.org/core/volume | "23"xsd:string |
| http://purl.uniprot.org/citations/8679110 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/8679110 |
| http://purl.uniprot.org/citations/8679110 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/8679110 |
| http://purl.uniprot.org/citations/8679110 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/8679110 |
| http://purl.uniprot.org/citations/8679110 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/8679110 |
| http://purl.uniprot.org/citations/8679110 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/8679110 |