| http://purl.uniprot.org/citations/8694776 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8694776 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8694776 | http://www.w3.org/2000/01/rdf-schema#comment | "Two enzymes in the methionine salvage pathway, 5-methylthioribose kinase (MTR kinase) and 5'-methylthioadenosine/ S-adenosylhomocysteine nucleosidase (MTA/SAH nucleosidase) were purified from Klebsiella pneumoniae. Chromatography using a novel 5'-(p-aminophenyl)thioadenosine/5-(p-aminophenyl)thioribose affinity matrix allowed the binding and selective elution of each of the enzymes in pure form. The molecular mass, substrate kinetics and N-terminal amino acid sequences were characterized for each of the enzymes. Purified MTR kinase exhibits an apparent molecular mass of 46-50 kDa by SDS/PAGE and S200HR chromatography, and has a Km for MTR of 12.2 microM. Homogeneous MTA/SAH nucleosidase displays a molecular mass of 26.5 kDa by SDS/PAGE, and a Km for MTA of 8.7 microM. Comparisons of the N-terminal sequences obtained for each of the enzymes with protein-sequence databases failed to reveal any significant sequence similarities to known proteins. However, the amino acid sequence obtained for the nucleosidase did share a high degree of sequence similarity with the putative translation product of an open reading frame in Escherichia coli, thus providing a tentative identification of this gene as encoding an MTA/SAH nucleosidase."xsd:string |
| http://purl.uniprot.org/citations/8694776 | http://purl.org/dc/terms/identifier | "doi:10.1042/bj3170285"xsd:string |
| http://purl.uniprot.org/citations/8694776 | http://purl.org/dc/terms/identifier | "doi:10.1042/bj3170285"xsd:string |
| http://purl.uniprot.org/citations/8694776 | http://purl.uniprot.org/core/author | "Tower P.A."xsd:string |
| http://purl.uniprot.org/citations/8694776 | http://purl.uniprot.org/core/author | "Tower P.A."xsd:string |
| http://purl.uniprot.org/citations/8694776 | http://purl.uniprot.org/core/author | "Cornell K.A."xsd:string |
| http://purl.uniprot.org/citations/8694776 | http://purl.uniprot.org/core/author | "Cornell K.A."xsd:string |
| http://purl.uniprot.org/citations/8694776 | http://purl.uniprot.org/core/author | "Riscoe M.K."xsd:string |
| http://purl.uniprot.org/citations/8694776 | http://purl.uniprot.org/core/author | "Riscoe M.K."xsd:string |
| http://purl.uniprot.org/citations/8694776 | http://purl.uniprot.org/core/author | "Winter R.W."xsd:string |
| http://purl.uniprot.org/citations/8694776 | http://purl.uniprot.org/core/author | "Winter R.W."xsd:string |
| http://purl.uniprot.org/citations/8694776 | http://purl.uniprot.org/core/date | "1996"xsd:gYear |
| http://purl.uniprot.org/citations/8694776 | http://purl.uniprot.org/core/date | "1996"xsd:gYear |
| http://purl.uniprot.org/citations/8694776 | http://purl.uniprot.org/core/name | "Biochem. J."xsd:string |
| http://purl.uniprot.org/citations/8694776 | http://purl.uniprot.org/core/name | "Biochem. J."xsd:string |
| http://purl.uniprot.org/citations/8694776 | http://purl.uniprot.org/core/pages | "285-290"xsd:string |
| http://purl.uniprot.org/citations/8694776 | http://purl.uniprot.org/core/pages | "285-290"xsd:string |
| http://purl.uniprot.org/citations/8694776 | http://purl.uniprot.org/core/title | "Affinity purification of 5-methylthioribose kinase and 5-methylthioadenosine/S-adenosylhomocysteine nucleosidase from Klebsiella pneumoniae."xsd:string |
| http://purl.uniprot.org/citations/8694776 | http://purl.uniprot.org/core/title | "Affinity purification of 5-methylthioribose kinase and 5-methylthioadenosine/S-adenosylhomocysteine nucleosidase from Klebsiella pneumoniae."xsd:string |
| http://purl.uniprot.org/citations/8694776 | http://purl.uniprot.org/core/volume | "317"xsd:string |
| http://purl.uniprot.org/citations/8694776 | http://purl.uniprot.org/core/volume | "317"xsd:string |
| http://purl.uniprot.org/citations/8694776 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/8694776 |
| http://purl.uniprot.org/citations/8694776 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/8694776 |