| http://purl.uniprot.org/citations/8703044 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8703044 | http://www.w3.org/2000/01/rdf-schema#comment | "We have previously proposed a molecular interaction between the liver factors that bind to the cyclic AMP response element (CRE) and CCAAT sites of the fibronectin (FN) gene based on the following evidence: (i) the close spacing of 20 base pairs between CRE and CCAAT elements is conserved in the FN genes from rats, mice, and humans; (ii) footprinting competitions showed that CRE oligonucleotides are able to detach both liver factors; (iii) CCAAT binding and transcriptional activity of liver extracts are reduced when the distance between the CRE and CCAAT elements is increased; and (iv) CCAAT-binding is stimulated by the addition of a liver extract fraction containing the CRE-binding factor ATF-2. This report provides binding and immunochemical evidence that nuclear factor I (CTF/NF-I) and CP1 (NF-Y or CBF) are the only liver factors that bind to the -150 CCAAT element of the FN gene, forming distinct complexes. We show that these factors bind less efficiently to the CCAAT site of a FN promoter in which the -170 CRE has been disrupted by site-directed mutagenesis and that each element contributes positively to the liver transcriptional activity assessed in vitro with a G-less cassette construct and in vivo by transfection of hepatoma cells with CAT constructs. Furthermore, using a method that combines UV cross-linking and immunoprecipitation, we show that antibodies specific to ATF-2 are able to specifically precipitate protein-protein-DNA complexes containing NF-I and CP1. This simple method preserves weak macromolecular interactions, avoiding the disruptive electrophoresis conditions of gel mobility shifts assays."xsd:string |
| http://purl.uniprot.org/citations/8703044 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.271.36.22271"xsd:string |
| http://purl.uniprot.org/citations/8703044 | http://purl.uniprot.org/core/author | "Kornblihtt A.R."xsd:string |
| http://purl.uniprot.org/citations/8703044 | http://purl.uniprot.org/core/author | "Alonso C.R."xsd:string |
| http://purl.uniprot.org/citations/8703044 | http://purl.uniprot.org/core/author | "Pesce C.G."xsd:string |
| http://purl.uniprot.org/citations/8703044 | http://purl.uniprot.org/core/date | "1996"xsd:gYear |
| http://purl.uniprot.org/citations/8703044 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/8703044 | http://purl.uniprot.org/core/pages | "22271-22279"xsd:string |
| http://purl.uniprot.org/citations/8703044 | http://purl.uniprot.org/core/title | "The CCAAT-binding proteins CP1 and NF-I cooperate with ATF-2 in the transcription of the fibronectin gene."xsd:string |
| http://purl.uniprot.org/citations/8703044 | http://purl.uniprot.org/core/volume | "271"xsd:string |
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