http://purl.uniprot.org/citations/8706824 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/8706824 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/8706824 | http://www.w3.org/2000/01/rdf-schema#comment | "Escherichia coli H(+)-ATPase subunit a is a hydrophobic F0 subunit. To investigate the topology of the subunit in the membrane, we prepared site-specific polyclonal antibodies against amino-terminal (Ser-3 to Leu-16), middle loop (Lys-167 to Gln-181), and carboxyl-terminal (Thr-259 to His-271) peptide segments. Enzyme-linked immunosorbent assay revealed that these antibodies specifically reacted with subunit a of inside-out membrane vesicles, but not with that of right-side-out spheroplasts. Full reactivity appeared when spheroplasts were disrupted with Triton X-100 (0.5%) or by sonication. These results suggest that at least parts of the three peptide segments of subunit a face the cytoplasm. Based on these observations, we propose a novel transmembrane topology of subunit a."xsd:string |
http://purl.uniprot.org/citations/8706824 | http://purl.org/dc/terms/identifier | "doi:10.1016/0014-5793(96)00621-7"xsd:string |
http://purl.uniprot.org/citations/8706824 | http://purl.org/dc/terms/identifier | "doi:10.1016/0014-5793(96)00621-7"xsd:string |
http://purl.uniprot.org/citations/8706824 | http://purl.uniprot.org/core/author | "Moriyama Y."xsd:string |
http://purl.uniprot.org/citations/8706824 | http://purl.uniprot.org/core/author | "Moriyama Y."xsd:string |
http://purl.uniprot.org/citations/8706824 | http://purl.uniprot.org/core/author | "Yamada H."xsd:string |
http://purl.uniprot.org/citations/8706824 | http://purl.uniprot.org/core/author | "Yamada H."xsd:string |
http://purl.uniprot.org/citations/8706824 | http://purl.uniprot.org/core/author | "Maeda M."xsd:string |
http://purl.uniprot.org/citations/8706824 | http://purl.uniprot.org/core/author | "Maeda M."xsd:string |
http://purl.uniprot.org/citations/8706824 | http://purl.uniprot.org/core/author | "Futai M."xsd:string |
http://purl.uniprot.org/citations/8706824 | http://purl.uniprot.org/core/author | "Futai M."xsd:string |
http://purl.uniprot.org/citations/8706824 | http://purl.uniprot.org/core/date | "1996"xsd:gYear |
http://purl.uniprot.org/citations/8706824 | http://purl.uniprot.org/core/date | "1996"xsd:gYear |
http://purl.uniprot.org/citations/8706824 | http://purl.uniprot.org/core/name | "FEBS Lett."xsd:string |
http://purl.uniprot.org/citations/8706824 | http://purl.uniprot.org/core/name | "FEBS Lett."xsd:string |
http://purl.uniprot.org/citations/8706824 | http://purl.uniprot.org/core/pages | "34-38"xsd:string |
http://purl.uniprot.org/citations/8706824 | http://purl.uniprot.org/core/pages | "34-38"xsd:string |
http://purl.uniprot.org/citations/8706824 | http://purl.uniprot.org/core/title | "Transmembrane topology of Escherichia coli H(+)-ATPase (ATP synthase) subunit a."xsd:string |
http://purl.uniprot.org/citations/8706824 | http://purl.uniprot.org/core/title | "Transmembrane topology of Escherichia coli H(+)-ATPase (ATP synthase) subunit a."xsd:string |
http://purl.uniprot.org/citations/8706824 | http://purl.uniprot.org/core/volume | "390"xsd:string |
http://purl.uniprot.org/citations/8706824 | http://purl.uniprot.org/core/volume | "390"xsd:string |
http://purl.uniprot.org/citations/8706824 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/8706824 |
http://purl.uniprot.org/citations/8706824 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/8706824 |