| http://purl.uniprot.org/citations/8709956 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8709956 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8709956 | http://www.w3.org/2000/01/rdf-schema#comment | "A technique that allows for easy identification of transformants of Neisseria gonorrhoeae in the absence of selective pressure has been developed. A suicide vector that contains a gonococcal DNA uptake sequence was constructed to aid in DNA uptake. In this transformation procedure, a limiting number of cells is incubated with an excess amount of DNA, and the mixture is plated onto a non-selective medium. At least 20% of the resulting colonies contained cells that had been transformed. This strategy was utilized to construct specific deletions of the S.N goI, II, IV, V and VII restriction-modification (R/M) genes. All five deletions were successfully incorporated into the chromosome of FA19, producing strain JUG029. Strain JUG029 could be transformed with non-methylated plasmid DNA while strain FA19 could not be transformed with such DNA. The development of a simple, non-selective transformation technique, coupled with the construction of a strain that is more permissive for DNA-mediated transformation, will aid in genetic manipulations of the gonococcus."xsd:string |
| http://purl.uniprot.org/citations/8709956 | http://purl.org/dc/terms/identifier | "doi:10.1007/s004380050196"xsd:string |
| http://purl.uniprot.org/citations/8709956 | http://purl.org/dc/terms/identifier | "doi:10.1007/bf02173639"xsd:string |
| http://purl.uniprot.org/citations/8709956 | http://purl.uniprot.org/core/author | "Stein D.C."xsd:string |
| http://purl.uniprot.org/citations/8709956 | http://purl.uniprot.org/core/author | "Stein D.C."xsd:string |
| http://purl.uniprot.org/citations/8709956 | http://purl.uniprot.org/core/author | "Gunn J.S."xsd:string |
| http://purl.uniprot.org/citations/8709956 | http://purl.uniprot.org/core/author | "Gunn J.S."xsd:string |
| http://purl.uniprot.org/citations/8709956 | http://purl.uniprot.org/core/date | "1996"xsd:gYear |
| http://purl.uniprot.org/citations/8709956 | http://purl.uniprot.org/core/date | "1996"xsd:gYear |
| http://purl.uniprot.org/citations/8709956 | http://purl.uniprot.org/core/name | "Mol. Gen. Genet."xsd:string |
| http://purl.uniprot.org/citations/8709956 | http://purl.uniprot.org/core/name | "Mol Gen Genet"xsd:string |
| http://purl.uniprot.org/citations/8709956 | http://purl.uniprot.org/core/pages | "509-517"xsd:string |
| http://purl.uniprot.org/citations/8709956 | http://purl.uniprot.org/core/pages | "509-517"xsd:string |
| http://purl.uniprot.org/citations/8709956 | http://purl.uniprot.org/core/title | "Use of a non-selective transformation technique to construct a multiply restriction/modification-deficient mutant of Neisseria gonorrhoeae."xsd:string |
| http://purl.uniprot.org/citations/8709956 | http://purl.uniprot.org/core/title | "Use of a non-selective transformation technique to construct a multiply restriction/modification-deficient mutant of Neisseria gonorrhoeae."xsd:string |
| http://purl.uniprot.org/citations/8709956 | http://purl.uniprot.org/core/volume | "251"xsd:string |
| http://purl.uniprot.org/citations/8709956 | http://purl.uniprot.org/core/volume | "251"xsd:string |
| http://purl.uniprot.org/citations/8709956 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/8709956 |
| http://purl.uniprot.org/citations/8709956 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/8709956 |
| http://purl.uniprot.org/citations/8709956 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/8709956 |
| http://purl.uniprot.org/citations/8709956 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/8709956 |
| http://purl.uniprot.org/uniprot/Q79CC3 | http://purl.uniprot.org/core/citation | http://purl.uniprot.org/citations/8709956 |
| http://purl.uniprot.org/uniprot/Q59604 | http://purl.uniprot.org/core/citation | http://purl.uniprot.org/citations/8709956 |