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| http://purl.uniprot.org/citations/8733591 | http://www.w3.org/2000/01/rdf-schema#comment | "1. The coding sequence of the P2Y1-purinoceptor was cloned from a human genomic library. 2. The open reading frame encodes a protein of 373 amino acids that is 83% identical to the previously cloned chick and turkey P2Y1-purinoceptor and is > or = 95% homologous to the recently cloned rat, mouse, and bovine P2Y1-purinoceptors. 3. The human P2Y1-purinoceptor was stably expressed in 1321N1 human astrocytoma cells using a retroviral vector. Although the P2Y1-purinoceptor agonist, 2MeSATP, had no effect on inositol phosphate accumulation in cells infected with the P2Y1-purinoceptor virus. No effect of 2MeSATP on cyclic AMP accumulation was observed in P2Y1-receptor-expressing 1321N1 cells. 4. The pharmacological selectively of 18 purinoceptor agonists was established for the expressed human P2Y1-purinoceptor. 2MeSATp was more potent than ATP but less potent than 2MeSADP. ADP also was more potent than ATP. A similar maximal effect was observed with most agonists tested. However, alpha, beta-MeATP had no effect and 3'-NH2-3'-deoxyATP and A2P4 were partial agonists. The order of potency of agonists for activation of the turkey P2Y1-purinoceptor, also stably expressed in 1321N1 cells, was identical to that observed for the human P2Y1-purinoceptor. 5. C6 glioma cells express a P2Y-purinoceptor that inhibits adenylyl cyclase but does not activate phospholipase C. Expression of the human P2Y1-purinoceptor in C6 cells conferred 2MeSATP-stimulated inositol lipid hydrolysis to these cells. The phospholipase C-activating human P2Y1-purinoceptor could be delineated from the endogenous P2Y-purinoceptor of C6 glioma cells by use of the P2-purinoceptor antagonist, PPADS, which blocks the P2Y1-purinoceptor but does not block the endogenous P2Y-purinoceptor of C6 cells. P2-purinoceptor agonists also exhibited differential selectivities for activation of these two P2Y-purinoceptors."xsd:string |
| http://purl.uniprot.org/citations/8733591 | http://purl.org/dc/terms/identifier | "doi:10.1111/j.1476-5381.1996.tb15381.x"xsd:string |
| http://purl.uniprot.org/citations/8733591 | http://purl.uniprot.org/core/author | "Li Q."xsd:string |
| http://purl.uniprot.org/citations/8733591 | http://purl.uniprot.org/core/author | "Boyer J.L."xsd:string |
| http://purl.uniprot.org/citations/8733591 | http://purl.uniprot.org/core/author | "Nicholas R.A."xsd:string |
| http://purl.uniprot.org/citations/8733591 | http://purl.uniprot.org/core/author | "Harden T.K."xsd:string |
| http://purl.uniprot.org/citations/8733591 | http://purl.uniprot.org/core/author | "Schachter J.B."xsd:string |
| http://purl.uniprot.org/citations/8733591 | http://purl.uniprot.org/core/date | "1996"xsd:gYear |
| http://purl.uniprot.org/citations/8733591 | http://purl.uniprot.org/core/name | "Br J Pharmacol"xsd:string |
| http://purl.uniprot.org/citations/8733591 | http://purl.uniprot.org/core/pages | "167-173"xsd:string |
| http://purl.uniprot.org/citations/8733591 | http://purl.uniprot.org/core/title | "Second messenger cascade specificity and pharmacological selectivity of the human P2Y1-purinoceptor."xsd:string |
| http://purl.uniprot.org/citations/8733591 | http://purl.uniprot.org/core/volume | "118"xsd:string |
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