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| Subject | Predicate | Object |
|---|---|---|
| http://purl.uniprot.org/citations/8752339 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8752339 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8752339 | http://www.w3.org/2000/01/rdf-schema#comment | "Klebsiella species express a family of structurally related lipopolysaccharide O antigens which share a common backbone known as D-galactan I. Serotype specificity results from modification of D-galactan I by addition of domains of altered structure or by substitution with O-acetyl and/or alpha-D-Galp side groups with various linkages and stoichiometries. In the prototype, Klebsiella serotype O1, the his-linked rfb gene cluster is required for synthesis of D-galactan I, but genes conferring serotype specificity are unlinked. The D-galactan I part of the O polysaccharide is O acetylated in Klebsiella serotype O8. By cloning the rfb region from Klebsiella serotype O8 and analyzing the O polysaccharide synthesized in Escherichia coli K-12 hosts, we show that, like rfbO1, the rfbO8 region directs formation of unmodified D-galactan I. The rfbAB genes encode an ATP-binding cassette transporter required for export of polymeric D-galactan I across the plasma membrane prior to completion of the lipopolysaccharide molecule by ligation of the O polysaccharide to lipid A-core. Complementation experiments show that the rfbAB gene products in serotypes O1 and O8 are functionally equivalent and interchangeable. Hybridization experiments and physical mapping of the rfb regions in related Klebsiella serotypes suggest the existence of shared rfb genes with a common organization. However, despite the functional equivalence of these rfb gene clusters, at least three distinct clonal groups were detected in different Klebsiella species and subspecies, on the basis of Southern hybridization experiments carried out under high-stringency conditions. The clonal groups cannot be predicted by features of the O-antigen structure. To examine the relationships in more detail, the complete nucleotide sequence of the serotype O8 rfb cluster was determined and compared with that of the serotype O1 prototype. The nucleotide sequences for the six rfb genes showed variations in moles percent G+C values and in the values for nucleotide sequence identity, which ranged from 66.9 to 79.7%. The predicted polypeptides ranged from 64.3% identity (78.4% total similarity) to 94.3% identity (98.0% similarity). The results presented here are not consistent with dissemination of the Klebsiella D-galactan I rfb genes through recent lateral transfer events."xsd:string |
| http://purl.uniprot.org/citations/8752339 | http://purl.org/dc/terms/identifier | "doi:10.1128/jb.178.17.5205-5214.1996"xsd:string |
| http://purl.uniprot.org/citations/8752339 | http://purl.org/dc/terms/identifier | "doi:10.1128/jb.178.17.5205-5214.1996"xsd:string |
| http://purl.uniprot.org/citations/8752339 | http://purl.uniprot.org/core/author | "Whitfield C."xsd:string |
| http://purl.uniprot.org/citations/8752339 | http://purl.uniprot.org/core/author | "Whitfield C."xsd:string |
| http://purl.uniprot.org/citations/8752339 | http://purl.uniprot.org/core/author | "Kelly R.F."xsd:string |
| http://purl.uniprot.org/citations/8752339 | http://purl.uniprot.org/core/author | "Kelly R.F."xsd:string |
| http://purl.uniprot.org/citations/8752339 | http://purl.uniprot.org/core/date | "1996"xsd:gYear |
| http://purl.uniprot.org/citations/8752339 | http://purl.uniprot.org/core/date | "1996"xsd:gYear |
| http://purl.uniprot.org/citations/8752339 | http://purl.uniprot.org/core/name | "J. Bacteriol."xsd:string |
| http://purl.uniprot.org/citations/8752339 | http://purl.uniprot.org/core/name | "J. Bacteriol."xsd:string |
| http://purl.uniprot.org/citations/8752339 | http://purl.uniprot.org/core/pages | "5205-5214"xsd:string |
| http://purl.uniprot.org/citations/8752339 | http://purl.uniprot.org/core/pages | "5205-5214"xsd:string |
| http://purl.uniprot.org/citations/8752339 | http://purl.uniprot.org/core/title | "Clonally diverse rfb gene clusters are involved in expression of a family of related D-galactan O antigens in Klebsiella species."xsd:string |
| http://purl.uniprot.org/citations/8752339 | http://purl.uniprot.org/core/title | "Clonally diverse rfb gene clusters are involved in expression of a family of related D-galactan O antigens in Klebsiella species."xsd:string |
| http://purl.uniprot.org/citations/8752339 | http://purl.uniprot.org/core/volume | "178"xsd:string |
| http://purl.uniprot.org/citations/8752339 | http://purl.uniprot.org/core/volume | "178"xsd:string |
| http://purl.uniprot.org/citations/8752339 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/8752339 |
| http://purl.uniprot.org/citations/8752339 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/8752339 |
| http://purl.uniprot.org/citations/8752339 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/8752339 |
| http://purl.uniprot.org/citations/8752339 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/8752339 |
| http://purl.uniprot.org/uniprot/Q48480 | http://purl.uniprot.org/core/citation | http://purl.uniprot.org/citations/8752339 |
| http://purl.uniprot.org/uniprot/Q48479 | http://purl.uniprot.org/core/citation | http://purl.uniprot.org/citations/8752339 |