| http://purl.uniprot.org/citations/8798539 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8798539 | http://www.w3.org/2000/01/rdf-schema#comment | "Cdc42 and Rac1 have been implicated in the regulation of various cell functions such as cell morphology, polarity, and cell proliferation. We have partially purified a Cdc42- and Rac1-associated protein with molecular mass of about 170 kDa (p170) from bovine brain cytosol. This protein interacted with guanosine 5'-(3-O-thio)triphosphate (GTPgammaS).glutathione S-transferase (GST)-Cdc42 and GTPgammaS++.GST-Rac1 but not with the GDP.GST-Cdc42, GDP.GST-Rac1, or GTPgammaS.GST-RhoA). We identified p170 as an IQGAP, which is originally identified as a putative Ras GTPase-activating protein. Recombinant IQGAP specifically interacted with GTPgammaS.Cdc42 and GTPgammaS.Rac1. The C-terminal fragment of IQGAP was responsible for their interactions. IQGAP was specifically immunoprecipitated with dominant-active Cdc42(Val12) or Rac1(Val12) from the COS7 cells expressing Cdc42(Val12) or Rac1(Val12), respectively. Immunofluorescence analysis revealed that IQGAP was accumulated at insulin- or Rac1-induced membrane ruffling areas. This accumulation of IQGAP was blocked by the microinjection of the dominant-negative Rac1(Asn17) or Cdc42(Asn17). Moreover, IQGAP was accumulated at the cell-cell junction in MDCK cells, where alpha-catenin and ZO-1 were localized. These results suggest that IQGAP is a novel target molecule for Cdc42 and Rac1."xsd:string |
| http://purl.uniprot.org/citations/8798539 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.271.38.23363"xsd:string |
| http://purl.uniprot.org/citations/8798539 | http://purl.uniprot.org/core/author | "Iwamatsu A."xsd:string |
| http://purl.uniprot.org/citations/8798539 | http://purl.uniprot.org/core/author | "Nomura N."xsd:string |
| http://purl.uniprot.org/citations/8798539 | http://purl.uniprot.org/core/author | "Kaibuchi K."xsd:string |
| http://purl.uniprot.org/citations/8798539 | http://purl.uniprot.org/core/author | "Kuroda S."xsd:string |
| http://purl.uniprot.org/citations/8798539 | http://purl.uniprot.org/core/author | "Kobayashi K."xsd:string |
| http://purl.uniprot.org/citations/8798539 | http://purl.uniprot.org/core/author | "Fukata M."xsd:string |
| http://purl.uniprot.org/citations/8798539 | http://purl.uniprot.org/core/author | "Nakafuku M."xsd:string |
| http://purl.uniprot.org/citations/8798539 | http://purl.uniprot.org/core/date | "1996"xsd:gYear |
| http://purl.uniprot.org/citations/8798539 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/8798539 | http://purl.uniprot.org/core/pages | "23363-23367"xsd:string |
| http://purl.uniprot.org/citations/8798539 | http://purl.uniprot.org/core/title | "Identification of IQGAP as a putative target for the small GTPases, Cdc42 and Rac1."xsd:string |
| http://purl.uniprot.org/citations/8798539 | http://purl.uniprot.org/core/volume | "271"xsd:string |
| http://purl.uniprot.org/citations/8798539 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/8798539 |
| http://purl.uniprot.org/citations/8798539 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/8798539 |
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| http://purl.uniprot.org/uniprot/#_A6NGB9-mappedCitation-8798539 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/8798539 |