Results
Your Query
▶
▶
Subject | Predicate | Object |
---|---|---|
http://purl.uniprot.org/citations/8808920 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/8808920 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/8808920 | http://www.w3.org/2000/01/rdf-schema#comment | "The heat shock response of Bradyrhizobium japonicum is controlled by a complex network involving two known regulatory systems. While some heat shock genes are controlled by a highly conserved inverted-repeat structure (CIRCE), others depend on a sigma 32-type heat shock sigma factor. Using Western blot (immunoblot) analysis, we confirmed the presence of a sigma 32-like protein in B. japonicum and defined its induction pattern after heat shock. A B. japonicum rpoH-like gene (rpoH1) was cloned by complementation of an Escherichia coli strain lacking sigma 32. A knockout mutation in rpoH1 did not abolish sigma 32 production in B. japonicum, and the rpoH1 mutant showed the wild-type growth phenotype, suggesting the presence of multiple rpoH homologs in this bacterium. Further characterization of the rpoH1 gene region revealed that the rpoH1 gene is located in a heat shock gene cluster together with the previously characterized groESL1 operon and three genes encoding small heat shock proteins in the following arrangement: groES1, groEL1, hspA, rpoH1, hspB, and hspC. Three heat-inducible promoters are responsible for transcription of the six genes as three bicistronic operons. A sigma 32-dependent promoter has previously been described upstream of the groESL1 operon. Although the hspA-rpoH1 and hspBC operons were clearly heat inducible, they were preceded by sigma 70-like promoters. Interestingly, a stretch of about 100 bp between the transcription start site and the start codon of the first gene in each of these two operons was nearly identical, making it a candidate for a regulatory element potentially allowing heat shock induction of sigma 70-dependent promoters."xsd:string |
http://purl.uniprot.org/citations/8808920 | http://purl.org/dc/terms/identifier | "doi:10.1128/jb.178.18.5337-5346.1996"xsd:string |
http://purl.uniprot.org/citations/8808920 | http://purl.org/dc/terms/identifier | "doi:10.1128/jb.178.18.5337-5346.1996"xsd:string |
http://purl.uniprot.org/citations/8808920 | http://purl.uniprot.org/core/author | "Hennecke H."xsd:string |
http://purl.uniprot.org/citations/8808920 | http://purl.uniprot.org/core/author | "Hennecke H."xsd:string |
http://purl.uniprot.org/citations/8808920 | http://purl.uniprot.org/core/author | "Fischer H.-M."xsd:string |
http://purl.uniprot.org/citations/8808920 | http://purl.uniprot.org/core/author | "Fischer H.-M."xsd:string |
http://purl.uniprot.org/citations/8808920 | http://purl.uniprot.org/core/author | "Narberhaus F."xsd:string |
http://purl.uniprot.org/citations/8808920 | http://purl.uniprot.org/core/author | "Narberhaus F."xsd:string |
http://purl.uniprot.org/citations/8808920 | http://purl.uniprot.org/core/author | "Weiglhofer W."xsd:string |
http://purl.uniprot.org/citations/8808920 | http://purl.uniprot.org/core/author | "Weiglhofer W."xsd:string |
http://purl.uniprot.org/citations/8808920 | http://purl.uniprot.org/core/author | "Fischer H.M."xsd:string |
http://purl.uniprot.org/citations/8808920 | http://purl.uniprot.org/core/date | "1996"xsd:gYear |
http://purl.uniprot.org/citations/8808920 | http://purl.uniprot.org/core/date | "1996"xsd:gYear |
http://purl.uniprot.org/citations/8808920 | http://purl.uniprot.org/core/name | "J. Bacteriol."xsd:string |
http://purl.uniprot.org/citations/8808920 | http://purl.uniprot.org/core/name | "J. Bacteriol."xsd:string |
http://purl.uniprot.org/citations/8808920 | http://purl.uniprot.org/core/pages | "5337-5346"xsd:string |
http://purl.uniprot.org/citations/8808920 | http://purl.uniprot.org/core/pages | "5337-5346"xsd:string |
http://purl.uniprot.org/citations/8808920 | http://purl.uniprot.org/core/title | "The Bradyrhizobium japonicum rpoH1 gene encoding a sigma 32-like protein is part of a unique heat shock gene cluster together with groESL1 and three small heat shock genes."xsd:string |
http://purl.uniprot.org/citations/8808920 | http://purl.uniprot.org/core/title | "The Bradyrhizobium japonicum rpoH1 gene encoding a sigma 32-like protein is part of a unique heat shock gene cluster together with groESL1 and three small heat shock genes."xsd:string |
http://purl.uniprot.org/citations/8808920 | http://purl.uniprot.org/core/volume | "178"xsd:string |
http://purl.uniprot.org/citations/8808920 | http://purl.uniprot.org/core/volume | "178"xsd:string |
http://purl.uniprot.org/citations/8808920 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/8808920 |