http://purl.uniprot.org/citations/8809036 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/8809036 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/8809036 | http://www.w3.org/2000/01/rdf-schema#comment | "Rapid Ca2+ efflux from intracellular stores during cardiac muscle excitation-contraction coupling is mediated by the ryanodine-sensitive calcium-release channel, a large homotetrameric complex present in the sarcoplasmic reticulum. We report here the identification, primary structure and topological analysis of the ryanodine receptor-calcium release channel from human cardiac muscle (hRyR-2). Consistent with sedimentation and immunoblotting studies on the hRyR-2 protein, sequence analysis of ten overlapping cDNA clones reveals an open reading frame of 14901 nucleotides encoding a protein of 4967 amino acid residues with a predicted molecular mass of 564 569 Da for hRyR-2. In-frame insertions corresponding to eight and ten amino acid residues were found in two of the ten cDNAs isolated, suggesting that novel, alternatively spliced transcripts of the hRyR-2 gene might exist. Six hydrophobic stretches, which are present within the hRyR-2 C-terminal 500 amino acids and are conserved in all RyR sequences, may be involved in forming the transmembrane domain that constitutes the Ca(2+)-conducting pathway, in agreement with competitive ELISA studies with a RyR-2-specific antibody. Sequence alignment of hRyR-2 with other RyR isoforms indicates a high level of overall identity within the RyR family, with the exception of two important regions that exhibit substantial variability. Phylogenetic analysis suggests that the RyR-2 isoform diverged from a single ancestral gene before the RyR-1 and RyR-3 isoforms to form a distinct branch of the RyR family tree."xsd:string |
http://purl.uniprot.org/citations/8809036 | http://purl.org/dc/terms/identifier | "doi:10.1042/bj3180477"xsd:string |
http://purl.uniprot.org/citations/8809036 | http://purl.org/dc/terms/identifier | "doi:10.1042/bj3180477"xsd:string |
http://purl.uniprot.org/citations/8809036 | http://purl.uniprot.org/core/author | "Lai F.A."xsd:string |
http://purl.uniprot.org/citations/8809036 | http://purl.uniprot.org/core/author | "Lai F.A."xsd:string |
http://purl.uniprot.org/citations/8809036 | http://purl.uniprot.org/core/author | "Allen P.D."xsd:string |
http://purl.uniprot.org/citations/8809036 | http://purl.uniprot.org/core/author | "Allen P.D."xsd:string |
http://purl.uniprot.org/citations/8809036 | http://purl.uniprot.org/core/author | "Walsh M.B."xsd:string |
http://purl.uniprot.org/citations/8809036 | http://purl.uniprot.org/core/author | "Walsh M.B."xsd:string |
http://purl.uniprot.org/citations/8809036 | http://purl.uniprot.org/core/author | "Shevchenko V.I."xsd:string |
http://purl.uniprot.org/citations/8809036 | http://purl.uniprot.org/core/author | "Shevchenko V.I."xsd:string |
http://purl.uniprot.org/citations/8809036 | http://purl.uniprot.org/core/author | "Bertrand B.M.A."xsd:string |
http://purl.uniprot.org/citations/8809036 | http://purl.uniprot.org/core/author | "Bertrand B.M.A."xsd:string |
http://purl.uniprot.org/citations/8809036 | http://purl.uniprot.org/core/author | "Tunwell R.E.A."xsd:string |
http://purl.uniprot.org/citations/8809036 | http://purl.uniprot.org/core/author | "Tunwell R.E.A."xsd:string |
http://purl.uniprot.org/citations/8809036 | http://purl.uniprot.org/core/author | "Wickenden C."xsd:string |
http://purl.uniprot.org/citations/8809036 | http://purl.uniprot.org/core/author | "Wickenden C."xsd:string |
http://purl.uniprot.org/citations/8809036 | http://purl.uniprot.org/core/date | "1996"xsd:gYear |
http://purl.uniprot.org/citations/8809036 | http://purl.uniprot.org/core/date | "1996"xsd:gYear |
http://purl.uniprot.org/citations/8809036 | http://purl.uniprot.org/core/name | "Biochem. J."xsd:string |
http://purl.uniprot.org/citations/8809036 | http://purl.uniprot.org/core/name | "Biochem. J."xsd:string |
http://purl.uniprot.org/citations/8809036 | http://purl.uniprot.org/core/pages | "477-487"xsd:string |
http://purl.uniprot.org/citations/8809036 | http://purl.uniprot.org/core/pages | "477-487"xsd:string |