| http://purl.uniprot.org/citations/8824310 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8824310 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8824310 | http://www.w3.org/2000/01/rdf-schema#comment | "Caenorhabditis elegans UNC-18 protein, homologous to yeast Sec1p, is important in neurotransmitter release, because the unc-18 mutation leads to severe paralysis and presynaptic acetylcholine (ACh) accumulation. To examine the functional conservation in mammals, we tried to isolate unc-18 isoforms from mouse and human brain cDNA libraries and obtained two classes of isoforms-neural genes and ubiquitous genes. Neural genes were identical to Munc-18 (also known as n-Sec1 or rbSec1), identified in rat and bovine brains as a syntaxin-binding protein. According to "Munc-18" terminology, we call the neural genes Munc-18-1 and the ubiquitous genes Munc-18-3. These mammalian isoforms exhibit 58% (Munc-18-1) and 42-43% (Munc-18-3) amino acid sequence identity with UNC-18. Next, we constructed transgenic unc-18 mutants to test biological activity of mouse Munc-18-1 and Munc-18-3 under the control of C. elegans unc-18 promoter. Munc-18-1 compensates for severe locomotion disability and cholinergic defects, e.g., abnormal sensitivities to cholinesterase inhibitors and cholinergic receptor agonists in unc-18 mutants, but Munc-18-3 fails. These data suggest that Munc-18-1 and C. elegans unc-18 may play positive roles in ACh release and that the molecular mechanism of neuronal regulated secretion has been partially conserved from nematodes to mammals."xsd:string |
| http://purl.uniprot.org/citations/8824310 | http://purl.org/dc/terms/identifier | "doi:10.1523/jneurosci.16-21-06695.1996"xsd:string |
| http://purl.uniprot.org/citations/8824310 | http://purl.org/dc/terms/identifier | "doi:10.1523/jneurosci.16-21-06695.1996"xsd:string |
| http://purl.uniprot.org/citations/8824310 | http://purl.uniprot.org/core/author | "Gengyo-Ando K."xsd:string |
| http://purl.uniprot.org/citations/8824310 | http://purl.uniprot.org/core/author | "Gengyo-Ando K."xsd:string |
| http://purl.uniprot.org/citations/8824310 | http://purl.uniprot.org/core/author | "Ikawa Y."xsd:string |
| http://purl.uniprot.org/citations/8824310 | http://purl.uniprot.org/core/author | "Ikawa Y."xsd:string |
| http://purl.uniprot.org/citations/8824310 | http://purl.uniprot.org/core/author | "Kitayama H."xsd:string |
| http://purl.uniprot.org/citations/8824310 | http://purl.uniprot.org/core/author | "Kitayama H."xsd:string |
| http://purl.uniprot.org/citations/8824310 | http://purl.uniprot.org/core/author | "Mukaida M."xsd:string |
| http://purl.uniprot.org/citations/8824310 | http://purl.uniprot.org/core/author | "Mukaida M."xsd:string |
| http://purl.uniprot.org/citations/8824310 | http://purl.uniprot.org/core/date | "1996"xsd:gYear |
| http://purl.uniprot.org/citations/8824310 | http://purl.uniprot.org/core/date | "1996"xsd:gYear |
| http://purl.uniprot.org/citations/8824310 | http://purl.uniprot.org/core/name | "J. Neurosci."xsd:string |
| http://purl.uniprot.org/citations/8824310 | http://purl.uniprot.org/core/name | "J. Neurosci."xsd:string |
| http://purl.uniprot.org/citations/8824310 | http://purl.uniprot.org/core/pages | "6695-6702"xsd:string |
| http://purl.uniprot.org/citations/8824310 | http://purl.uniprot.org/core/pages | "6695-6702"xsd:string |
| http://purl.uniprot.org/citations/8824310 | http://purl.uniprot.org/core/title | "A murine neural-specific homolog corrects cholinergic defects in Caenorhabditis elegans unc-18 mutants."xsd:string |
| http://purl.uniprot.org/citations/8824310 | http://purl.uniprot.org/core/title | "A murine neural-specific homolog corrects cholinergic defects in Caenorhabditis elegans unc-18 mutants."xsd:string |
| http://purl.uniprot.org/citations/8824310 | http://purl.uniprot.org/core/volume | "16"xsd:string |
| http://purl.uniprot.org/citations/8824310 | http://purl.uniprot.org/core/volume | "16"xsd:string |
| http://purl.uniprot.org/citations/8824310 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/8824310 |
| http://purl.uniprot.org/citations/8824310 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/8824310 |