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| Subject | Predicate | Object |
|---|---|---|
| http://purl.uniprot.org/citations/8837440 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8837440 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8837440 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Citation |
| http://purl.uniprot.org/citations/8837440 | http://www.w3.org/2000/01/rdf-schema#comment | "A cDNA expression library of Trichoderma reesei RutC-30 was constructed in the yeast Saccharomyces cerevisiae. Two genes, abf1 and bxl1, were isolated by screening the yeast library for extracellular alpha-L-arabinofuranosidase activity with the substrate p-nitrophenyl-alpha-L-arabinofuranoside. The genes abf1 and bxl1 encode 500 and 758 amino acids, respectively, including the signal sequences. The deduced amino acid sequence of ABFI displays high-level similarity to the alpha-L-arabinofuranosidase B of Aspergillus niger, and the two can form a new family of glycosyl hydrolases. The deduced amino acid sequence of BXLI shows similarities to the beta-glucosidases grouped in family 3. The yeast-produced enzymes were tested for enzymatic activities against different substrates. ABFI released L-arabinose from p-nitrophenyl-alpha-L-arabinofuranoside and arabinoxylans and showed some beta-xylosidase activity toward p-nitrophenyl-beta-D-xylopyranoside. BXLI did not release L-arabinose from arabinoxylan. It showed alpha-L-arabinofuranosidase, alpha-L-arabinopyranosidase, and beta-xylosidase activities against p-nitrophenyl-alpha-L-arabinofuranosidase, p-nitrophenyl-alpha-L-arabinopyranoside, and p-nitrophenyl-beta-D-xylopyranoside, respectively, with the last activity being the highest. It was also able to hydrolyze xylobiose and slowly release xylose from polymeric xylan. ABFI and BXLI correspond to a previously purified alpha-L-arabinofuranosidase and a beta-xylosidase from T. reesei, respectively, as confirmed by partial amino acid sequencing of the Trichoderma-produced enzymes. Both enzymes produced in yeasts displayed hydrolytic properties similar to those of the corresponding enzymes purified from T. reesei."xsd:string |
| http://purl.uniprot.org/citations/8837440 | http://purl.org/dc/terms/identifier | "doi:10.1128/aem.62.10.3840-3846.1996"xsd:string |
| http://purl.uniprot.org/citations/8837440 | http://purl.org/dc/terms/identifier | "doi:10.1128/aem.62.10.3840-3846.1996"xsd:string |
| http://purl.uniprot.org/citations/8837440 | http://purl.org/dc/terms/identifier | "doi:10.1128/aem.62.10.3840-3846.1996"xsd:string |
| http://purl.uniprot.org/citations/8837440 | http://purl.uniprot.org/core/author | "Penttilae M."xsd:string |
| http://purl.uniprot.org/citations/8837440 | http://purl.uniprot.org/core/author | "Penttilae M."xsd:string |
| http://purl.uniprot.org/citations/8837440 | http://purl.uniprot.org/core/author | "Margolles-Clark E."xsd:string |
| http://purl.uniprot.org/citations/8837440 | http://purl.uniprot.org/core/author | "Margolles-Clark E."xsd:string |
| http://purl.uniprot.org/citations/8837440 | http://purl.uniprot.org/core/author | "Nakari-Setaelae T."xsd:string |
| http://purl.uniprot.org/citations/8837440 | http://purl.uniprot.org/core/author | "Nakari-Setaelae T."xsd:string |
| http://purl.uniprot.org/citations/8837440 | http://purl.uniprot.org/core/author | "Tenkanen M."xsd:string |
| http://purl.uniprot.org/citations/8837440 | http://purl.uniprot.org/core/author | "Tenkanen M."xsd:string |
| http://purl.uniprot.org/citations/8837440 | http://purl.uniprot.org/core/date | "1996"xsd:gYear |
| http://purl.uniprot.org/citations/8837440 | http://purl.uniprot.org/core/date | "1996"xsd:gYear |
| http://purl.uniprot.org/citations/8837440 | http://purl.uniprot.org/core/name | "Appl. Environ. Microbiol."xsd:string |
| http://purl.uniprot.org/citations/8837440 | http://purl.uniprot.org/core/name | "Appl. Environ. Microbiol."xsd:string |
| http://purl.uniprot.org/citations/8837440 | http://purl.uniprot.org/core/pages | "3840-3846"xsd:string |
| http://purl.uniprot.org/citations/8837440 | http://purl.uniprot.org/core/pages | "3840-3846"xsd:string |
| http://purl.uniprot.org/citations/8837440 | http://purl.uniprot.org/core/title | "Cloning of genes encoding alpha-L-arabinofuranosidase and beta-xylosidase from Trichoderma reesei by expression in Saccharomyces cerevisiae."xsd:string |
| http://purl.uniprot.org/citations/8837440 | http://purl.uniprot.org/core/title | "Cloning of genes encoding alpha-L-arabinofuranosidase and beta-xylosidase from Trichoderma reesei by expression in Saccharomyces cerevisiae."xsd:string |
| http://purl.uniprot.org/citations/8837440 | http://purl.uniprot.org/core/volume | "62"xsd:string |
| http://purl.uniprot.org/citations/8837440 | http://purl.uniprot.org/core/volume | "62"xsd:string |