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http://purl.uniprot.org/citations/8855261http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/8855261http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/8855261http://www.w3.org/2000/01/rdf-schema#comment"Membrane depolarization of NG108 cells gives rapid (< 5 min) activation of Ca2+/calmodulin-dependent protein kinase IV (CaM-KIV), as well as activation of c-Jun N-terminal kinase (JNK). To investigate whether the Ca2+-dependent activation of mitogen-activated protein kinases (ERK, JNK, and p38) might be mediated by the CaM kinase cascade, we have transfected PC12 cells, which lack CaM-KIV, with constitutively active mutants of CaM kinase kinase and/or CaM-KIV (CaM-KKc and CaM-KIVc, respectively). In the absence of depolarization, CaM-KKc transfection had no effect on Elk-dependent transcription of a luciferase reporter gene, whereas CaM-KIVc alone or in combination with CaM-KKc gave 7-to 10-fold and 60-to 80-fold stimulations, respectively, which were blocked by mitogen-activated protein (MAP) kinase phosphatase cotransfection. When epitope-tagged constructs of MAP kinases were co-transfected with CaM-KKc plus CaM-KIVc, the immunoprecipitated MAP kinases were activated 2-fold (ERK-2) and 7-to 10-fold (JNK-1 and p38). The JNK and p38 pathways were further investigated using specific c-Jun or ATF2-dependent transcriptional assays. We found that c-Jun/ATF2-dependent transcriptions were enhanced 7-to 10-fold by CaM-KIVc and 20-to 30-fold by CaM-KKc plus CaM-KIVc. In the case of the Jun-dependent transcription, this effect was not due to direct phosphorylation of c-Jun by activated CaM-KIV, since transcription was blocked by a dominant-negative JNK and by two MAP kinase phosphatases. Mutation of the phosphorylation site (Thr196) in CaM-KIV, which mediates its activation by CaM-KIV kinase, prevented activation of Elk-1, c-Jun, and ATF2 by the CaM kinase cascade. These results establish a new Ca2+-dependent mechanism for regulating MAP kinase pathways and resultant transcription."xsd:string
http://purl.uniprot.org/citations/8855261http://purl.org/dc/terms/identifier"doi:10.1073/pnas.93.20.10803"xsd:string
http://purl.uniprot.org/citations/8855261http://purl.org/dc/terms/identifier"doi:10.1073/pnas.93.20.10803"xsd:string
http://purl.uniprot.org/citations/8855261http://purl.uniprot.org/core/author"Davis R.J."xsd:string
http://purl.uniprot.org/citations/8855261http://purl.uniprot.org/core/author"Davis R.J."xsd:string
http://purl.uniprot.org/citations/8855261http://purl.uniprot.org/core/author"Tokumitsu H."xsd:string
http://purl.uniprot.org/citations/8855261http://purl.uniprot.org/core/author"Tokumitsu H."xsd:string
http://purl.uniprot.org/citations/8855261http://purl.uniprot.org/core/author"Soderling T.R."xsd:string
http://purl.uniprot.org/citations/8855261http://purl.uniprot.org/core/author"Soderling T.R."xsd:string
http://purl.uniprot.org/citations/8855261http://purl.uniprot.org/core/author"Enslen H."xsd:string
http://purl.uniprot.org/citations/8855261http://purl.uniprot.org/core/author"Enslen H."xsd:string
http://purl.uniprot.org/citations/8855261http://purl.uniprot.org/core/author"Stork P.J."xsd:string
http://purl.uniprot.org/citations/8855261http://purl.uniprot.org/core/author"Stork P.J."xsd:string
http://purl.uniprot.org/citations/8855261http://purl.uniprot.org/core/date"1996"xsd:gYear
http://purl.uniprot.org/citations/8855261http://purl.uniprot.org/core/date"1996"xsd:gYear
http://purl.uniprot.org/citations/8855261http://purl.uniprot.org/core/name"Proc. Natl. Acad. Sci. U.S.A."xsd:string
http://purl.uniprot.org/citations/8855261http://purl.uniprot.org/core/name"Proc. Natl. Acad. Sci. U.S.A."xsd:string
http://purl.uniprot.org/citations/8855261http://purl.uniprot.org/core/pages"10803-10808"xsd:string
http://purl.uniprot.org/citations/8855261http://purl.uniprot.org/core/pages"10803-10808"xsd:string
http://purl.uniprot.org/citations/8855261http://purl.uniprot.org/core/title"Regulation of mitogen-activated protein kinases by a calcium/calmodulin-dependent protein kinase cascade."xsd:string
http://purl.uniprot.org/citations/8855261http://purl.uniprot.org/core/title"Regulation of mitogen-activated protein kinases by a calcium/calmodulin-dependent protein kinase cascade."xsd:string
http://purl.uniprot.org/citations/8855261http://purl.uniprot.org/core/volume"93"xsd:string
http://purl.uniprot.org/citations/8855261http://purl.uniprot.org/core/volume"93"xsd:string
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