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| http://purl.uniprot.org/citations/8855951 | http://www.w3.org/2000/01/rdf-schema#comment | "Primer extension and RNase protection analyses of the rat beta 2-adrenergic receptor (beta 2AR) gene identify two transcription start points at -64 and -220 nt, respectively. Transient transfections of putative promoter/pCAT constructs into DDT1 MF-2 cells indicate that fragments -36 to -100 (PI) and -186 to -312 (P2) are sufficient to promote transcription, whereas -911 to -1122 contains a negative regulatory element(s). RNase protection analysis of the 3' untranslated region (3'-UTR) indicates the presence of two transcripts with 3'-UTR of 111 and 604 nt exclusive of the poly(A+) tails. Northern blots of beta 2AR mRNA using full-length and partial cDNA probes indicate that a major 2.2 kb and a minor 1.6 kb species arise from the use of alternative promoters as well as different polyadenylation signals. DNase I footprinting and DNA mobility shift assays (DMSA) using rat liver nuclear extracts identify a number of transcription factors binding to sequence elements within or upstream from P1 and P2, including Spl, CRE, CPl, AP-2, NF-1, NF-kappa B, and C/EBP. Supershift assays using antibodies against C/EBP alpha and C/EBP beta and mutational analyses indicate that the protein binding to the C/EBP consensus recognition site at -925 to -933 is C/EBP alpha. The activity of promoter/CAT constructs containing the C/EBP recognition site is significantly decreased by cotransfection of C/EBP alpha but not C/EBP alpha but not C/EBP beta into either DDT1 MF-2 cells or primary rat hepatocytes. Partial hepatectomy causes a transient decrease in C/EBP alpha, as measured by DMSA, and an increase in beta 2 AR mRNA levels and rate of transcription in the remnant liver. Thus, derepression via C/EBP alpha is likely involved in the up-regulation of beta 2AR in the regenerating rat liver."xsd:string |
| http://purl.uniprot.org/citations/8855951 | http://purl.org/dc/terms/identifier | "doi:10.1021/bi960844o"xsd:string |
| http://purl.uniprot.org/citations/8855951 | http://purl.uniprot.org/core/author | "Jiang L."xsd:string |
| http://purl.uniprot.org/citations/8855951 | http://purl.uniprot.org/core/author | "Gao B."xsd:string |
| http://purl.uniprot.org/citations/8855951 | http://purl.uniprot.org/core/author | "Kunos G."xsd:string |
| http://purl.uniprot.org/citations/8855951 | http://purl.uniprot.org/core/date | "1996"xsd:gYear |
| http://purl.uniprot.org/citations/8855951 | http://purl.uniprot.org/core/name | "Biochemistry"xsd:string |
| http://purl.uniprot.org/citations/8855951 | http://purl.uniprot.org/core/pages | "13136-13146"xsd:string |
| http://purl.uniprot.org/citations/8855951 | http://purl.uniprot.org/core/title | "DNA elements and protein factors involved in the transcription of the beta 2-adrenergic receptor gene in rat liver. The negative regulatory role of C/EBP alpha."xsd:string |
| http://purl.uniprot.org/citations/8855951 | http://purl.uniprot.org/core/volume | "35"xsd:string |
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