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http://purl.uniprot.org/citations/8902629http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/8902629http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/8902629http://www.w3.org/2000/01/rdf-schema#comment"Human medium-chain enoyl-CoA hydratase was purified from liver, because we noticed the presence of a high medium-chain enoyl-CoA hydratase activity in human skin fibroblasts catalyzed by an enzyme different from the known enzymes catalyzing the enoyl-CoA hydratase reaction. Two enzyme preparations were obtained. One of them, preparation I, consisted of 46-kDa polypeptide, and its molecular mass was estimated to be 86 kDa. The other, preparation II, consisted of a major 77-kDa polypeptide and minor smaller polypeptides including 46-kDa polypeptide. The molecular mass of preparation II was 154 kDa. Both enzyme preparations catalyzed reversible dehydration of medium-chain D-3-hydroxyacyl-CoA to 2-trans-enoyl-CoA, but did not react with L-3-hydroxyacyl-CoA. Catalytic properties and immunochemical reactivities of these enzyme preparations were nearly the same. The cross-reactive material to the antibody was confirmed to be in peroxisomes by immunohistochemical study of cultured human skin fibroblasts."xsd:string
http://purl.uniprot.org/citations/8902629http://purl.org/dc/terms/identifier"doi:10.1093/oxfordjournals.jbchem.a021458"xsd:string
http://purl.uniprot.org/citations/8902629http://purl.org/dc/terms/identifier"doi:10.1093/oxfordjournals.jbchem.a021458"xsd:string
http://purl.uniprot.org/citations/8902629http://purl.uniprot.org/core/author"Hashimoto T."xsd:string
http://purl.uniprot.org/citations/8902629http://purl.uniprot.org/core/author"Hashimoto T."xsd:string
http://purl.uniprot.org/citations/8902629http://purl.uniprot.org/core/author"Kobayashi A."xsd:string
http://purl.uniprot.org/citations/8902629http://purl.uniprot.org/core/author"Kobayashi A."xsd:string
http://purl.uniprot.org/citations/8902629http://purl.uniprot.org/core/author"Fukushima H."xsd:string
http://purl.uniprot.org/citations/8902629http://purl.uniprot.org/core/author"Fukushima H."xsd:string
http://purl.uniprot.org/citations/8902629http://purl.uniprot.org/core/author"Matsuura H."xsd:string
http://purl.uniprot.org/citations/8902629http://purl.uniprot.org/core/author"Matsuura H."xsd:string
http://purl.uniprot.org/citations/8902629http://purl.uniprot.org/core/author"Jiang L.L."xsd:string
http://purl.uniprot.org/citations/8902629http://purl.uniprot.org/core/author"Jiang L.L."xsd:string
http://purl.uniprot.org/citations/8902629http://purl.uniprot.org/core/date"1996"xsd:gYear
http://purl.uniprot.org/citations/8902629http://purl.uniprot.org/core/date"1996"xsd:gYear
http://purl.uniprot.org/citations/8902629http://purl.uniprot.org/core/name"J. Biochem."xsd:string
http://purl.uniprot.org/citations/8902629http://purl.uniprot.org/core/name"J. Biochem."xsd:string
http://purl.uniprot.org/citations/8902629http://purl.uniprot.org/core/pages"624-632"xsd:string
http://purl.uniprot.org/citations/8902629http://purl.uniprot.org/core/pages"624-632"xsd:string
http://purl.uniprot.org/citations/8902629http://purl.uniprot.org/core/title"Purification and properties of human D-3-hydroxyacyl-CoA dehydratase: medium-chain enoyl-CoA hydratase is D-3-hydroxyacyl-CoA dehydratase."xsd:string
http://purl.uniprot.org/citations/8902629http://purl.uniprot.org/core/title"Purification and properties of human D-3-hydroxyacyl-CoA dehydratase: medium-chain enoyl-CoA hydratase is D-3-hydroxyacyl-CoA dehydratase."xsd:string
http://purl.uniprot.org/citations/8902629http://purl.uniprot.org/core/volume"120"xsd:string
http://purl.uniprot.org/citations/8902629http://purl.uniprot.org/core/volume"120"xsd:string