| http://purl.uniprot.org/citations/8913454 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8913454 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8913454 | http://www.w3.org/2000/01/rdf-schema#comment | "We have analyzed by gene amplification and sequencing mutations in the quinolone resistance-determining regions of the gyrA, gyrB, and parC genes of fluoroquinolone-resistant Streptococcus pneumoniae mutants obtained during therapy or in vitro. Mutations leading to substitutions in ParC were detected in the two mutants obtained in vivo, BM4203-R (substitution of a histidine for an aspartate at position 84 [Asp-84-->His]; Staphylococcus aureus coordinates) and BM4204-R (Ser-80-->Phe), and in two mutants obtained in vitro (Ser-80-->Tyr). An additional mutant obtained in vitro, BM4205-R3, displayed a higher level of fluoroquinolone resistance and had a mutation in gyrA leading to a Ser-84-->Phe change. We could not detect any mutation in the three remaining mutants obtained in vitro. Total DNA from BM4203-R, BM4204-R, and BM4205-R3 was used to transform S. pneumoniae CP1000 by selection on fluoroquinolones. For the parC mutants, transformants with phenotypes indistinguishable from those of the donors were obtained at frequencies (5 x 10(-3) to 8 x 10(-3)) compatible with monogenic transformation. By contrast, transformants were obtained at a low frequency (4 x 10(-5)), compatible with the transformation of two independent genes, for the gyrA mutant. Resistant transformants of CP1000 were also obtained with an amplified fragment of parC from BM4203-R and BM4204-R but not with a gyrA fragment from BM4205-R3. All transformants had mutations identical to those in the donors. These data strongly suggest that ParC is the primary target for fluoroquinolones in S. pneumoniae and that BM4205-R3 is resistant to higher levels of the drugs following the acquisition of two mutations, including one in gyrA."xsd:string |
| http://purl.uniprot.org/citations/8913454 | http://purl.org/dc/terms/identifier | "doi:10.1128/aac.40.11.2505"xsd:string |
| http://purl.uniprot.org/citations/8913454 | http://purl.org/dc/terms/identifier | "doi:10.1128/aac.40.11.2505"xsd:string |
| http://purl.uniprot.org/citations/8913454 | http://purl.uniprot.org/core/author | "Courvalin P."xsd:string |
| http://purl.uniprot.org/citations/8913454 | http://purl.uniprot.org/core/author | "Courvalin P."xsd:string |
| http://purl.uniprot.org/citations/8913454 | http://purl.uniprot.org/core/author | "Perichon B."xsd:string |
| http://purl.uniprot.org/citations/8913454 | http://purl.uniprot.org/core/author | "Perichon B."xsd:string |
| http://purl.uniprot.org/citations/8913454 | http://purl.uniprot.org/core/author | "Tankovic J."xsd:string |
| http://purl.uniprot.org/citations/8913454 | http://purl.uniprot.org/core/author | "Tankovic J."xsd:string |
| http://purl.uniprot.org/citations/8913454 | http://purl.uniprot.org/core/author | "Duval J."xsd:string |
| http://purl.uniprot.org/citations/8913454 | http://purl.uniprot.org/core/author | "Duval J."xsd:string |
| http://purl.uniprot.org/citations/8913454 | http://purl.uniprot.org/core/date | "1996"xsd:gYear |
| http://purl.uniprot.org/citations/8913454 | http://purl.uniprot.org/core/date | "1996"xsd:gYear |
| http://purl.uniprot.org/citations/8913454 | http://purl.uniprot.org/core/name | "Antimicrob. Agents Chemother."xsd:string |
| http://purl.uniprot.org/citations/8913454 | http://purl.uniprot.org/core/name | "Antimicrob. Agents Chemother."xsd:string |
| http://purl.uniprot.org/citations/8913454 | http://purl.uniprot.org/core/pages | "2505-2510"xsd:string |
| http://purl.uniprot.org/citations/8913454 | http://purl.uniprot.org/core/pages | "2505-2510"xsd:string |
| http://purl.uniprot.org/citations/8913454 | http://purl.uniprot.org/core/title | "Contribution of mutations in gyrA and parC genes to fluoroquinolone resistance of mutants of Streptococcus pneumoniae obtained in vivo and in vitro."xsd:string |
| http://purl.uniprot.org/citations/8913454 | http://purl.uniprot.org/core/title | "Contribution of mutations in gyrA and parC genes to fluoroquinolone resistance of mutants of Streptococcus pneumoniae obtained in vivo and in vitro."xsd:string |
| http://purl.uniprot.org/citations/8913454 | http://purl.uniprot.org/core/volume | "40"xsd:string |
| http://purl.uniprot.org/citations/8913454 | http://purl.uniprot.org/core/volume | "40"xsd:string |
| http://purl.uniprot.org/citations/8913454 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/8913454 |
| http://purl.uniprot.org/citations/8913454 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/8913454 |