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| http://purl.uniprot.org/citations/8930895 | http://www.w3.org/2000/01/rdf-schema#comment | "During purification of recombinant Cdc6 expressed in yeast, we found that Cdc6 interacts with the critical cell cycle, cyclin-dependent protein kinase Cdc28. Cdc6 and Cdc28 can be coimmunoprecipitated from extracts, Cdc6 is retained on the Cdc28-binding matrix p13-agarose, and Cdc28 is retained on an affinity column charged with bacterially produced Cdc6. Cdc6, which is a phosphoprotein in vivo, contains five Cdc28 consensus sites and is a substrate of the Cdc28 kinase in vitro. Cdc6 also inhibits Cdc28 histone H1 kinase activity. Strikingly, Cdc6 interacts preferentially with B-type cyclin/Cdc28 complexes and not Cln/Cdc28 in log-phase cells. However, Cdc6 does not associate with Cdc28 when cells are blocked at the restrictive temperature in a cdc34 mutant, a point in the cell cycle when the B-type cyclin/Cdc28 inhibitor p40Sic1 accumulates and purified p40Sic1 inhibits the Cdc6/Cdc28 interaction. Deletion of the Cdc28 interaction domain from Cdc6 yields a protein that cannot support growth. However, when overproduced, the mutant protein can support growth. Furthermore, whereas overproduction of wild-type Cdc6 leads to growth inhibition and bud hyperpolarization, overproduction of the mutant protein supports growth at normal rates with normal morphology. Thus, the interaction may have a role in the essential function of Cdc6 in initiation and in restraining mitosis until replication is complete."xsd:string |
| http://purl.uniprot.org/citations/8930895 | http://purl.org/dc/terms/identifier | "doi:10.1091/mbc.7.11.1723"xsd:string |
| http://purl.uniprot.org/citations/8930895 | http://purl.uniprot.org/core/author | "Wang B."xsd:string |
| http://purl.uniprot.org/citations/8930895 | http://purl.uniprot.org/core/author | "Campbell J.L."xsd:string |
| http://purl.uniprot.org/citations/8930895 | http://purl.uniprot.org/core/author | "Jong A."xsd:string |
| http://purl.uniprot.org/citations/8930895 | http://purl.uniprot.org/core/author | "Elsasser S."xsd:string |
| http://purl.uniprot.org/citations/8930895 | http://purl.uniprot.org/core/author | "Lou F."xsd:string |
| http://purl.uniprot.org/citations/8930895 | http://purl.uniprot.org/core/date | "1996"xsd:gYear |
| http://purl.uniprot.org/citations/8930895 | http://purl.uniprot.org/core/name | "Mol Biol Cell"xsd:string |
| http://purl.uniprot.org/citations/8930895 | http://purl.uniprot.org/core/pages | "1723-1735"xsd:string |
| http://purl.uniprot.org/citations/8930895 | http://purl.uniprot.org/core/title | "Interaction between yeast Cdc6 protein and B-type cyclin/Cdc28 kinases."xsd:string |
| http://purl.uniprot.org/citations/8930895 | http://purl.uniprot.org/core/volume | "7"xsd:string |
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