| http://purl.uniprot.org/citations/8940068 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8940068 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8940068 | http://www.w3.org/2000/01/rdf-schema#comment | "Mammalian heat shock genes are regulated at the transcriptional level by heat shock factor-1 (HSF-1), a sequence-specific transcription factor. We have examined the role of serine phosphorylation of HSF-1 in the regulation of heat shock gene transcription. Our experiments show that mitogen-activated protein kinases (MAPKs) of the ERK-1 family phosphorylate HSF-1 on serine residues and repress the transcriptional activation of the heat shock protein 70B (HSP70B) promoter by HSF-1 in vivo. These effects of MAPK are transmitted through a specific serine residue (Ser-303) located in a proline-rich sequence within the transcriptional regulatory domain of human HSF-1. However, despite the importance of Ser-303 in transmitting the signal from the MAPK cascade to HSP70 transcription, there was no evidence that Ser-303 could be phosphorylated by MAPK in vitro, although an adjacent residue (Ser-307) was avidly phosphorylated by MAPK. Further studies revealed that Ser-303 is phosphorylated by glycogen synthase kinase 3 (GSK3) through a mechanism dependent on primary phosphorylation of Ser-307 by MAPK. Secondary phosphorylation of Ser-303 by GSK3 may thus repress the activity of HSF-1, and its requirement for priming by MAPK phosphorylation of Ser-307 provides a potential link between the MAPK cascade and HSF-1. Our experiments thus indicate that MAPK is a potent inhibitor of HSF-1 function and may be involved in repressing the heat shock response during normal growth and development and deactivating the heat shock response during recovery from stress."xsd:string |
| http://purl.uniprot.org/citations/8940068 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.271.48.30847"xsd:string |
| http://purl.uniprot.org/citations/8940068 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.271.48.30847"xsd:string |
| http://purl.uniprot.org/citations/8940068 | http://purl.uniprot.org/core/author | "Calderwood S.K."xsd:string |
| http://purl.uniprot.org/citations/8940068 | http://purl.uniprot.org/core/author | "Calderwood S.K."xsd:string |
| http://purl.uniprot.org/citations/8940068 | http://purl.uniprot.org/core/author | "Price B.D."xsd:string |
| http://purl.uniprot.org/citations/8940068 | http://purl.uniprot.org/core/author | "Price B.D."xsd:string |
| http://purl.uniprot.org/citations/8940068 | http://purl.uniprot.org/core/author | "Chu B."xsd:string |
| http://purl.uniprot.org/citations/8940068 | http://purl.uniprot.org/core/author | "Chu B."xsd:string |
| http://purl.uniprot.org/citations/8940068 | http://purl.uniprot.org/core/author | "Soncin F."xsd:string |
| http://purl.uniprot.org/citations/8940068 | http://purl.uniprot.org/core/author | "Soncin F."xsd:string |
| http://purl.uniprot.org/citations/8940068 | http://purl.uniprot.org/core/author | "Stevenson M.A."xsd:string |
| http://purl.uniprot.org/citations/8940068 | http://purl.uniprot.org/core/author | "Stevenson M.A."xsd:string |
| http://purl.uniprot.org/citations/8940068 | http://purl.uniprot.org/core/date | "1996"xsd:gYear |
| http://purl.uniprot.org/citations/8940068 | http://purl.uniprot.org/core/date | "1996"xsd:gYear |
| http://purl.uniprot.org/citations/8940068 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/8940068 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/8940068 | http://purl.uniprot.org/core/pages | "30847-30857"xsd:string |
| http://purl.uniprot.org/citations/8940068 | http://purl.uniprot.org/core/pages | "30847-30857"xsd:string |
| http://purl.uniprot.org/citations/8940068 | http://purl.uniprot.org/core/title | "Sequential phosphorylation by mitogen-activated protein kinase and glycogen synthase kinase 3 represses transcriptional activation by heat shock factor-1."xsd:string |
| http://purl.uniprot.org/citations/8940068 | http://purl.uniprot.org/core/title | "Sequential phosphorylation by mitogen-activated protein kinase and glycogen synthase kinase 3 represses transcriptional activation by heat shock factor-1."xsd:string |
| http://purl.uniprot.org/citations/8940068 | http://purl.uniprot.org/core/volume | "271"xsd:string |
| http://purl.uniprot.org/citations/8940068 | http://purl.uniprot.org/core/volume | "271"xsd:string |