| http://purl.uniprot.org/citations/8948598 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8948598 | http://www.w3.org/2000/01/rdf-schema#comment | "Dorsoventral polarity in the Drosophila embryo is established by a signaling pathway active on the ventral and ventrolateral surfaces of the embryo. Signal transduction via the protein kinase Pelle frees the Rel-related protein Dorsal from its cytoplasmic inhibitor Cactus, allowing Dorsal to translocate into ventral and ventrolateral nuclei and direct gene expression. Here, we show by immunochemical analyses that Pelle-mediated signaling induces the spatially graded degradation of Cactus. Using a tissue culture system which reconstitutes Pelle-dependent Cactus degradation, we show that a motif in Cactus resembling the sites of signal-dependent phosphorylation in the vertebrate homologs IkappaB-alpha and IkappaB-beta is essential for Pelle-induced Cactus degradation. Substitution of four serines within this motif with nonphosphorylatable alanine residues generated a mutant Cactus that still functions as a Dorsal inhibitor but is resistant to induced degradation. Injection of RNA encoding this altered form of Cactus has a dominant negative effect on establishment of dorsoventral polarity in the embryo. We conclude that dorsoventral signaling results in a Cactus concentration gradient and propose that signal-dependent phosphorylation directs the spatially regulated proteolysis of Cactus protein."xsd:string |
| http://purl.uniprot.org/citations/8948598 | http://purl.org/dc/terms/identifier | "doi:10.1006/dbio.1996.0308"xsd:string |
| http://purl.uniprot.org/citations/8948598 | http://purl.uniprot.org/core/author | "Karin M."xsd:string |
| http://purl.uniprot.org/citations/8948598 | http://purl.uniprot.org/core/author | "Wasserman S.A."xsd:string |
| http://purl.uniprot.org/citations/8948598 | http://purl.uniprot.org/core/author | "Allen J.L."xsd:string |
| http://purl.uniprot.org/citations/8948598 | http://purl.uniprot.org/core/author | "Galindo R.L."xsd:string |
| http://purl.uniprot.org/citations/8948598 | http://purl.uniprot.org/core/author | "Reach M."xsd:string |
| http://purl.uniprot.org/citations/8948598 | http://purl.uniprot.org/core/author | "Towb P."xsd:string |
| http://purl.uniprot.org/citations/8948598 | http://purl.uniprot.org/core/date | "1996"xsd:gYear |
| http://purl.uniprot.org/citations/8948598 | http://purl.uniprot.org/core/name | "Dev Biol"xsd:string |
| http://purl.uniprot.org/citations/8948598 | http://purl.uniprot.org/core/pages | "353-364"xsd:string |
| http://purl.uniprot.org/citations/8948598 | http://purl.uniprot.org/core/title | "A gradient of cactus protein degradation establishes dorsoventral polarity in the Drosophila embryo."xsd:string |
| http://purl.uniprot.org/citations/8948598 | http://purl.uniprot.org/core/volume | "180"xsd:string |
| http://purl.uniprot.org/citations/8948598 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/8948598 |
| http://purl.uniprot.org/citations/8948598 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/8948598 |
| http://purl.uniprot.org/uniprot/#_A0A0B4KHQ0-mappedCitation-8948598 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/8948598 |
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| http://purl.uniprot.org/uniprot/#_A0A0B4LGC1-mappedCitation-8948598 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/8948598 |
| http://purl.uniprot.org/uniprot/#_C7LA93-mappedCitation-8948598 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/8948598 |
| http://purl.uniprot.org/uniprot/#_E1JHK3-mappedCitation-8948598 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/8948598 |
| http://purl.uniprot.org/uniprot/#_M9PD95-mappedCitation-8948598 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/8948598 |
| http://purl.uniprot.org/uniprot/#_P10627-mappedCitation-8948598 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/8948598 |
| http://purl.uniprot.org/uniprot/#_O62589-mappedCitation-8948598 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/8948598 |