| http://purl.uniprot.org/citations/8972853 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8972853 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8972853 | http://www.w3.org/2000/01/rdf-schema#comment | "An interference assay has been devised in Schizosaccharomyces pombe to rapidly identify and clone genes involved in chromosome segregation. Random S.pombe cDNAs were overexpressed from an inducible promoter in a strain carrying an additional, non-essential minichromosome. Overexpression of cDNAs derived from four genes, two known (nda3+and ubc4+, encoding beta-tubulin and a ubiquitin conjugating enzyme, respectively) and two unknown, named mlo2+ and mlo3+ (missegregation & lethal when over expressed) caused phenotypes consistent with a failure to segregate chromosomes. Full overexpression of all four cDNAs was lethal. Cells overexpressing nda3+ and ubc4+ cDNAs arrested with condensed unsegregated chromosomes and cells overexpressing mlo2+ displayed an asymmetric distribution of nuclear chromatin. Sublethal levels of overexpression of nda3+, ubc4+ and mlo2+ cDNAs caused elevated rates of minichromosome loss. A third cDNA mlo3+, displayed no increase in the frequency of minichromosome loss at sublethal levels of overexpression but full overexpression caused a complete failure to segregate chromosomes. Our results confirm the assumption that beta-tubulin overexpression is lethal in S.pombe, implicate ubc4+ in the control of metaphase-anaphase transition in fission yeast and finally identify two new genes, mlo2+and mlo3+, likely to play an important role for chromosome transmission fidelity in mitosis."xsd:string |
| http://purl.uniprot.org/citations/8972853 | http://purl.org/dc/terms/identifier | "doi:10.1093/nar/24.23.4676"xsd:string |
| http://purl.uniprot.org/citations/8972853 | http://purl.org/dc/terms/identifier | "doi:10.1093/nar/24.23.4676"xsd:string |
| http://purl.uniprot.org/citations/8972853 | http://purl.uniprot.org/core/author | "Allshire R.C."xsd:string |
| http://purl.uniprot.org/citations/8972853 | http://purl.uniprot.org/core/author | "Allshire R.C."xsd:string |
| http://purl.uniprot.org/citations/8972853 | http://purl.uniprot.org/core/author | "Javerzat J.-P."xsd:string |
| http://purl.uniprot.org/citations/8972853 | http://purl.uniprot.org/core/author | "Javerzat J.-P."xsd:string |
| http://purl.uniprot.org/citations/8972853 | http://purl.uniprot.org/core/author | "Cranston G."xsd:string |
| http://purl.uniprot.org/citations/8972853 | http://purl.uniprot.org/core/author | "Cranston G."xsd:string |
| http://purl.uniprot.org/citations/8972853 | http://purl.uniprot.org/core/date | "1996"xsd:gYear |
| http://purl.uniprot.org/citations/8972853 | http://purl.uniprot.org/core/date | "1996"xsd:gYear |
| http://purl.uniprot.org/citations/8972853 | http://purl.uniprot.org/core/name | "Nucleic Acids Res."xsd:string |
| http://purl.uniprot.org/citations/8972853 | http://purl.uniprot.org/core/name | "Nucleic Acids Res."xsd:string |
| http://purl.uniprot.org/citations/8972853 | http://purl.uniprot.org/core/pages | "4676-4683"xsd:string |
| http://purl.uniprot.org/citations/8972853 | http://purl.uniprot.org/core/pages | "4676-4683"xsd:string |
| http://purl.uniprot.org/citations/8972853 | http://purl.uniprot.org/core/title | "Fission yeast genes which disrupt mitotic chromosome segregation when overexpressed."xsd:string |
| http://purl.uniprot.org/citations/8972853 | http://purl.uniprot.org/core/title | "Fission yeast genes which disrupt mitotic chromosome segregation when overexpressed."xsd:string |
| http://purl.uniprot.org/citations/8972853 | http://purl.uniprot.org/core/volume | "24"xsd:string |
| http://purl.uniprot.org/citations/8972853 | http://purl.uniprot.org/core/volume | "24"xsd:string |
| http://purl.uniprot.org/citations/8972853 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/8972853 |
| http://purl.uniprot.org/citations/8972853 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/8972853 |
| http://purl.uniprot.org/citations/8972853 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/8972853 |
| http://purl.uniprot.org/citations/8972853 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/8972853 |