http://purl.uniprot.org/citations/8973579 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/8973579 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/8973579 | http://www.w3.org/2000/01/rdf-schema#comment | "A phosphatidylinositol (PtdIns) 4-kinase cDNA cloned from a rat brain cDNA library encoded a protein of 816 amino acids with a calculated molecular mass of 91654 Da. This molecule contained a lipid-kinase-unique domain and a presumed lipid/ protein kinase homology domain that are found in other PtdIns 4-kinases and PtdIns 3-kinases. Furthermore, this kinase molecule had 43.3% shared identity with the presumed catalytic domain of yeast PtdIns 4-kinase, PtdInsK1, and the two molecules had a region of similarity that is not conserved in other lipid kinases. By examining PtdIns kinase activity in transfected COS-7 cells using epitope tag immunoprecipitation as well as conventional methods, the product PtdIns phosphate was identified as phosphatidylinositol 4-phosphate (PtdIns4P), but not phosphatidylinositol 3-phosphate (PtdIns3P). The PtdIns 4-kinase activity was recovered predominantly from the soluble fraction and the activity was markedly enhanced in the presence of Triton X-100 and was relatively insensitive to inhibition by adenosine. In addition, the PtdIns 4-kinase activity was completely inhibited in the presence of 10 microM wortmannin. When examined by epitope tag immunocytochemistry, the immunoreactivity for the PtdIns 4-kinase molecule was dominantly aggregated in a cytoplasmic region juxtaposed to the nuclei and was faintly but widely dispersed in the cytoplasm. By in situ hybridization analysis, the mRNA for PtdIns 4-kinase was expressed ubiquitously and was detected in most neurons throughout the grey matter of the brain, with higher expression intensity found in fetal than in adult brain."xsd:string |
http://purl.uniprot.org/citations/8973579 | http://purl.org/dc/terms/identifier | "doi:10.1042/bj3200643"xsd:string |
http://purl.uniprot.org/citations/8973579 | http://purl.org/dc/terms/identifier | "doi:10.1042/bj3200643"xsd:string |
http://purl.uniprot.org/citations/8973579 | http://purl.uniprot.org/core/author | "Kondo H."xsd:string |
http://purl.uniprot.org/citations/8973579 | http://purl.uniprot.org/core/author | "Kondo H."xsd:string |
http://purl.uniprot.org/citations/8973579 | http://purl.uniprot.org/core/author | "Goto K."xsd:string |
http://purl.uniprot.org/citations/8973579 | http://purl.uniprot.org/core/author | "Goto K."xsd:string |
http://purl.uniprot.org/citations/8973579 | http://purl.uniprot.org/core/author | "Nakagawa T."xsd:string |
http://purl.uniprot.org/citations/8973579 | http://purl.uniprot.org/core/author | "Nakagawa T."xsd:string |
http://purl.uniprot.org/citations/8973579 | http://purl.uniprot.org/core/date | "1996"xsd:gYear |
http://purl.uniprot.org/citations/8973579 | http://purl.uniprot.org/core/date | "1996"xsd:gYear |
http://purl.uniprot.org/citations/8973579 | http://purl.uniprot.org/core/name | "Biochem. J."xsd:string |
http://purl.uniprot.org/citations/8973579 | http://purl.uniprot.org/core/name | "Biochem. J."xsd:string |
http://purl.uniprot.org/citations/8973579 | http://purl.uniprot.org/core/pages | "643-649"xsd:string |
http://purl.uniprot.org/citations/8973579 | http://purl.uniprot.org/core/pages | "643-649"xsd:string |
http://purl.uniprot.org/citations/8973579 | http://purl.uniprot.org/core/title | "Cloning and characterization of a 92 kDa soluble phosphatidylinositol 4-kinase."xsd:string |
http://purl.uniprot.org/citations/8973579 | http://purl.uniprot.org/core/title | "Cloning and characterization of a 92 kDa soluble phosphatidylinositol 4-kinase."xsd:string |
http://purl.uniprot.org/citations/8973579 | http://purl.uniprot.org/core/volume | "320"xsd:string |
http://purl.uniprot.org/citations/8973579 | http://purl.uniprot.org/core/volume | "320"xsd:string |
http://purl.uniprot.org/citations/8973579 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/8973579 |
http://purl.uniprot.org/citations/8973579 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/8973579 |
http://purl.uniprot.org/citations/8973579 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/8973579 |
http://purl.uniprot.org/citations/8973579 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/8973579 |