| http://purl.uniprot.org/citations/9009282 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9009282 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9009282 | http://www.w3.org/2000/01/rdf-schema#comment | "Trypanosoma cruzi trypomastigotes are exquisitely resistant to the lytic effects of vertebrate complement, and this characteristic contributes to the survival of the parasites in the host bloodstream. Trypomastigotes avoid complement-mediated lysis by the production of a surface glycoprotein that inhibits the formation of the alternative and classical C3 convertase, thus preventing activation and amplification of the complement cascade at the parasite surface. We have developed a monoclonal antibody to the 160-kDa T. cruzi complement regulatory protein (CRP) and describe a one-step immunoaffinity purification procedure. The CRP was purified to homogeneity and subjected to amino-terminal peptide sequence analysis. Based on the protein sequence obtained, the CRP was identified as a member of a large family of trypomastigote-specific genes, and a complete cDNA was isolated and sequenced. The complete coding sequence was cloned in Escherichia coli, and antibodies raised against the full-length recombinant protein reacted specifically with a 160-kDa protein in trypomastigote membrane protein preparations as well as with native, purified CRP. Indirect immunofluorescence revealed that the protein is uniformly expressed at the cell surfaces of trypomastigotes."xsd:string |
| http://purl.uniprot.org/citations/9009282 | http://purl.org/dc/terms/identifier | "doi:10.1128/iai.65.2.349-357.1997"xsd:string |
| http://purl.uniprot.org/citations/9009282 | http://purl.uniprot.org/core/author | "Schrimpf J.E."xsd:string |
| http://purl.uniprot.org/citations/9009282 | http://purl.uniprot.org/core/author | "Schrimpf J.E."xsd:string |
| http://purl.uniprot.org/citations/9009282 | http://purl.uniprot.org/core/author | "Szabo M.J."xsd:string |
| http://purl.uniprot.org/citations/9009282 | http://purl.uniprot.org/core/author | "Szabo M.J."xsd:string |
| http://purl.uniprot.org/citations/9009282 | http://purl.uniprot.org/core/author | "Norris K.A."xsd:string |
| http://purl.uniprot.org/citations/9009282 | http://purl.uniprot.org/core/author | "Norris K.A."xsd:string |
| http://purl.uniprot.org/citations/9009282 | http://purl.uniprot.org/core/date | "1997"xsd:gYear |
| http://purl.uniprot.org/citations/9009282 | http://purl.uniprot.org/core/date | "1997"xsd:gYear |
| http://purl.uniprot.org/citations/9009282 | http://purl.uniprot.org/core/name | "Infect. Immun."xsd:string |
| http://purl.uniprot.org/citations/9009282 | http://purl.uniprot.org/core/name | "Infect Immun"xsd:string |
| http://purl.uniprot.org/citations/9009282 | http://purl.uniprot.org/core/pages | "349-357"xsd:string |
| http://purl.uniprot.org/citations/9009282 | http://purl.uniprot.org/core/pages | "349-357"xsd:string |
| http://purl.uniprot.org/citations/9009282 | http://purl.uniprot.org/core/title | "Identification of the gene family encoding the 160-kilodalton Trypanosoma cruzi complement regulatory protein."xsd:string |
| http://purl.uniprot.org/citations/9009282 | http://purl.uniprot.org/core/title | "Identification of the gene family encoding the 160-kilodalton Trypanosoma cruzi complement regulatory protein."xsd:string |
| http://purl.uniprot.org/citations/9009282 | http://purl.uniprot.org/core/volume | "65"xsd:string |
| http://purl.uniprot.org/citations/9009282 | http://purl.uniprot.org/core/volume | "65"xsd:string |
| http://purl.uniprot.org/citations/9009282 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/9009282 |
| http://purl.uniprot.org/citations/9009282 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/9009282 |
| http://purl.uniprot.org/citations/9009282 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/9009282 |
| http://purl.uniprot.org/citations/9009282 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/9009282 |
| http://purl.uniprot.org/uniprot/Q26889 | http://purl.uniprot.org/core/citation | http://purl.uniprot.org/citations/9009282 |