| http://purl.uniprot.org/citations/9015300 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9015300 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9015300 | http://www.w3.org/2000/01/rdf-schema#comment | "The localization of proteins to late-Golgi membranes (TGN) of Saccharomyces cerevisiae is conferred by targeting motifs containing aromatic residues in the cytosolic domains of these proteins. These signals could act by directing retrieval from a post-Golgi compartment or by preventing exit from the TGN. To investigate the mechanism of localization of yeast TGN proteins, we used the heterologous protein A-ALP (consisting of the cytosolic domain of dipeptidyl aminopeptidase A [DPAP A] fused to the transmembrane and luminal domains of the vacuolar protein alkaline phosphatase [ALP]), which localizes to the yeast TGN. Insertion of the aromatic residue-based TGN localization motif (FXFXD) of DPAP A into the cytosolic domain of ALP results in a protein that resides in the TGN. We demonstrate that the FXFXD motif confers Golgi localization through retrieval from a post-Golgi compartment by detecting a post-Golgi processed form of this protein in the TGN. We present an assay that uncouples retrieval-mediated Golgi localization from static retention-based localization, allowing measurement of the rate at which proteins exit the yeast TGN. We also demonstrate that the cytosolic domain of DPAP A contains additional information, separate from the retrieval motif, that slows exit from the TGN. We propose a model for DPAP A localization that involves two distinct mechanisms: one in which the FXFXD motif directs retrieval from a post-Golgi compartment, and a second that slows the rate at which DPAP A exits the TGN."xsd:string |
| http://purl.uniprot.org/citations/9015300 | http://purl.org/dc/terms/identifier | "doi:10.1083/jcb.136.2.287"xsd:string |
| http://purl.uniprot.org/citations/9015300 | http://purl.org/dc/terms/identifier | "doi:10.1083/jcb.136.2.287"xsd:string |
| http://purl.uniprot.org/citations/9015300 | http://purl.uniprot.org/core/author | "Stevens T.H."xsd:string |
| http://purl.uniprot.org/citations/9015300 | http://purl.uniprot.org/core/author | "Stevens T.H."xsd:string |
| http://purl.uniprot.org/citations/9015300 | http://purl.uniprot.org/core/author | "Bryant N.J."xsd:string |
| http://purl.uniprot.org/citations/9015300 | http://purl.uniprot.org/core/author | "Bryant N.J."xsd:string |
| http://purl.uniprot.org/citations/9015300 | http://purl.uniprot.org/core/date | "1997"xsd:gYear |
| http://purl.uniprot.org/citations/9015300 | http://purl.uniprot.org/core/date | "1997"xsd:gYear |
| http://purl.uniprot.org/citations/9015300 | http://purl.uniprot.org/core/name | "J. Cell Biol."xsd:string |
| http://purl.uniprot.org/citations/9015300 | http://purl.uniprot.org/core/name | "J. Cell Biol."xsd:string |
| http://purl.uniprot.org/citations/9015300 | http://purl.uniprot.org/core/pages | "287-297"xsd:string |
| http://purl.uniprot.org/citations/9015300 | http://purl.uniprot.org/core/pages | "287-297"xsd:string |
| http://purl.uniprot.org/citations/9015300 | http://purl.uniprot.org/core/title | "Two separate signals act independently to localize a yeast late Golgi membrane protein through a combination of retrieval and retention."xsd:string |
| http://purl.uniprot.org/citations/9015300 | http://purl.uniprot.org/core/title | "Two separate signals act independently to localize a yeast late Golgi membrane protein through a combination of retrieval and retention."xsd:string |
| http://purl.uniprot.org/citations/9015300 | http://purl.uniprot.org/core/volume | "136"xsd:string |
| http://purl.uniprot.org/citations/9015300 | http://purl.uniprot.org/core/volume | "136"xsd:string |
| http://purl.uniprot.org/citations/9015300 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/9015300 |
| http://purl.uniprot.org/citations/9015300 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/9015300 |
| http://purl.uniprot.org/citations/9015300 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/9015300 |
| http://purl.uniprot.org/citations/9015300 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/9015300 |
| http://purl.uniprot.org/uniprot/P40343 | http://purl.uniprot.org/core/citation | http://purl.uniprot.org/citations/9015300 |
| http://purl.uniprot.org/uniprot/P40343#attribution-986F6DAD04500FC5D4A359861115A652 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/9015300 |