| http://purl.uniprot.org/citations/9024699 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9024699 | http://www.w3.org/2000/01/rdf-schema#comment | "L-Selectin on neutrophils as well as inducible E- and P-selectin on endothelium are involved in the recruitment of neutrophils into inflamed tissue. Based on cell attachment assays, L-selectin was suggested to function as a carbohydrate presenting ligand for E- and P-selectin. However, previous affinity isolation experiments with an E-selectin-Ig fusion protein had failed to detect L-selectin among the isolated E-selectin ligands from mouse neutrophils. We show here that L-selectin from human neutrophils, in contrast to mouse neutrophils, can be affinity-isolated as a major ligand from total cell extracts using E-selectin-Ig as affinity probe. Binding of human L-selectin to E-selectin was direct, since purified L-selectin could be reprecipitated with E-selectin-Ig. Recognition of L-selectin was abolished by sialidase-treatment, required Ca2+, and was resistant to treatment with endoglycosidase F. Binding of L-selectin to a P-selectin-Ig fusion protein was not observed. In agreement with the biochemical data, the anti-L-selectin mAb DREG56 inhibited rolling of human neutrophils on immobilized E-selectin-Ig but not on P-selectin-Ig. No such inhibitory effect was seen with the anti-mouse L-selectin mAb MEL14 on mouse neutrophils. Rolling of E-selectin transfectants on purified and immobilized human L-selectin was inhibited by mAb DREG56. We conclude that L-selectin on human neutrophils is a major glycoprotein ligand among very few glycoproteins that can be isolated by an E-selectin affinity matrix. The clear difference between human and mouse L-selectin suggests that E-selectin-binding carbohydrate moieties are attached to different protein scaffolds in different species."xsd:string |
| http://purl.uniprot.org/citations/9024699 | http://purl.org/dc/terms/identifier | "doi:10.1083/jcb.136.3.707"xsd:string |
| http://purl.uniprot.org/citations/9024699 | http://purl.uniprot.org/core/author | "Zerwes H.G."xsd:string |
| http://purl.uniprot.org/citations/9024699 | http://purl.uniprot.org/core/author | "Vestweber D."xsd:string |
| http://purl.uniprot.org/citations/9024699 | http://purl.uniprot.org/core/author | "Steegmaier M."xsd:string |
| http://purl.uniprot.org/citations/9024699 | http://purl.uniprot.org/core/author | "Lenter M.C."xsd:string |
| http://purl.uniprot.org/citations/9024699 | http://purl.uniprot.org/core/author | "Borges E."xsd:string |
| http://purl.uniprot.org/citations/9024699 | http://purl.uniprot.org/core/author | "Blanks J.E."xsd:string |
| http://purl.uniprot.org/citations/9024699 | http://purl.uniprot.org/core/author | "Zollner O."xsd:string |
| http://purl.uniprot.org/citations/9024699 | http://purl.uniprot.org/core/date | "1997"xsd:gYear |
| http://purl.uniprot.org/citations/9024699 | http://purl.uniprot.org/core/name | "J Cell Biol"xsd:string |
| http://purl.uniprot.org/citations/9024699 | http://purl.uniprot.org/core/pages | "707-716"xsd:string |
| http://purl.uniprot.org/citations/9024699 | http://purl.uniprot.org/core/title | "L-selectin from human, but not from mouse neutrophils binds directly to E-selectin."xsd:string |
| http://purl.uniprot.org/citations/9024699 | http://purl.uniprot.org/core/volume | "136"xsd:string |
| http://purl.uniprot.org/citations/9024699 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/9024699 |
| http://purl.uniprot.org/citations/9024699 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/9024699 |
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| http://purl.uniprot.org/uniprot/#_B1B506-mappedCitation-9024699 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/9024699 |
| http://purl.uniprot.org/uniprot/#_B1B507-mappedCitation-9024699 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/9024699 |
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| http://purl.uniprot.org/uniprot/#_P18337-mappedCitation-9024699 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/9024699 |
| http://purl.uniprot.org/uniprot/#_Q3TCF3-mappedCitation-9024699 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/9024699 |
| http://purl.uniprot.org/uniprot/#_Q3TA36-mappedCitation-9024699 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/9024699 |