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http://purl.uniprot.org/citations/9025278http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/9025278http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/9025278http://www.w3.org/2000/01/rdf-schema#comment"Two monoclonal antibodies (mAbs) designated 144,H-3 (IgG2a) and 218,C-5 (IgM) were produced after immunization of mice with two different heat-treated and sonicated pneumococcal strains. Western blotting, with solubilized proteins from different bacterial genera and from mammalian lymphocytes, showed that both mAbs reacted with a protein of approximately 12 kDa in all 66 strains of eubacteria examined, representing 27 different species. The 12 kDa protein was isolated by immunoaffinity chromatography. Subsequent preparative Western blotting enabled N-terminal amino acid sequence analysis by microsequencing. A high degree of amino acid sequence similarity with eubacterial ribosomal proteins L7/L12 was demonstrated. One of the mAbs (144,H-3) also cross-reacted in Western blotting with a 43 kDa protein, but only from streptococci. The 43 kDa protein carrying the common streptococcal epitope was isolated and sequenced in the N-terminal region. A high degree of amino acid sequence identity was found to elongation factor Ts from Escherichia coli."xsd:string
http://purl.uniprot.org/citations/9025278http://purl.org/dc/terms/identifier"doi:10.1099/00221287-143-1-55"xsd:string
http://purl.uniprot.org/citations/9025278http://purl.org/dc/terms/identifier"doi:10.1099/00221287-143-1-55"xsd:string
http://purl.uniprot.org/citations/9025278http://purl.uniprot.org/core/author"Lopez R."xsd:string
http://purl.uniprot.org/citations/9025278http://purl.uniprot.org/core/author"Lopez R."xsd:string
http://purl.uniprot.org/citations/9025278http://purl.uniprot.org/core/author"Sletten K."xsd:string
http://purl.uniprot.org/citations/9025278http://purl.uniprot.org/core/author"Sletten K."xsd:string
http://purl.uniprot.org/citations/9025278http://purl.uniprot.org/core/author"Hoiby E.A."xsd:string
http://purl.uniprot.org/citations/9025278http://purl.uniprot.org/core/author"Hoiby E.A."xsd:string
http://purl.uniprot.org/citations/9025278http://purl.uniprot.org/core/author"Kolberg J."xsd:string
http://purl.uniprot.org/citations/9025278http://purl.uniprot.org/core/author"Kolberg J."xsd:string
http://purl.uniprot.org/citations/9025278http://purl.uniprot.org/core/date"1997"xsd:gYear
http://purl.uniprot.org/citations/9025278http://purl.uniprot.org/core/date"1997"xsd:gYear
http://purl.uniprot.org/citations/9025278http://purl.uniprot.org/core/name"Microbiology"xsd:string
http://purl.uniprot.org/citations/9025278http://purl.uniprot.org/core/name"Microbiology"xsd:string
http://purl.uniprot.org/citations/9025278http://purl.uniprot.org/core/pages"55-61"xsd:string
http://purl.uniprot.org/citations/9025278http://purl.uniprot.org/core/pages"55-61"xsd:string
http://purl.uniprot.org/citations/9025278http://purl.uniprot.org/core/title"Monoclonal antibodies against Streptococcus pneumoniae detect epitopes on eubacterial ribosomal proteins L7/L12 and on streptococcal elongation factor Ts."xsd:string
http://purl.uniprot.org/citations/9025278http://purl.uniprot.org/core/title"Monoclonal antibodies against Streptococcus pneumoniae detect epitopes on eubacterial ribosomal proteins L7/L12 and on streptococcal elongation factor Ts."xsd:string
http://purl.uniprot.org/citations/9025278http://purl.uniprot.org/core/volume"143"xsd:string
http://purl.uniprot.org/citations/9025278http://purl.uniprot.org/core/volume"143"xsd:string
http://purl.uniprot.org/citations/9025278http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/9025278
http://purl.uniprot.org/citations/9025278http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/9025278