| http://purl.uniprot.org/citations/9041391 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9041391 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9041391 | http://www.w3.org/2000/01/rdf-schema#comment | "Amplification of a 3-kb genome region from the RNA polymerase gene to the 3' poly(A) tail of small round-structured virus (SRSV) by reverse transcription-PCR (RT-PCR) has been difficult to achieve because of a stable secondary structure in a region between the RNA polymerase gene and the 5' end of the second open reading frame. We have developed a one-tube RT-PCR method to efficiently amplify this region. The method comprises three procedures: purification of poly(A)+ RNA from a starting RNA solution by oligo(dT)30 covalently linked to latex particles, buffer exchange, and continuous RT and PCR in a single tube containing all reaction components. The key elements of this method are (i) first-strand cDNA synthesis with the Superscript II version of RNase H-Moloney murine leukemia virus reverse transcriptase at 50 degrees C for 10 min by using the RNA-oligo(dT)30 hybrid on the latex particles as the template and primer, and (ii) PCR by Taq and Pwo DNA polymerases mixed together with a mixture of 12 phased oligo(dT)25 antisense primers. The detection threshold of the one-tube RT-PCR method was as little as 0.2 ng of the crude RNA used as the source of the template. Using this method, we obtained 3-kb products from 24 SRSV strains previously characterized into four genetic groups. These included 5 P1-A, 4 P1-B, 5 P2-A, and 10 P2-B strains. Because SRSVs have not yet been cultivated in vitro, this novel method should facilitate molecular characterization of SRSVs to provide a firm scientific foundation for improvements and refinements of SRSV diagnostics."xsd:string |
| http://purl.uniprot.org/citations/9041391 | http://purl.uniprot.org/core/name | "J. Clin. Microbiol."xsd:string |
| http://purl.uniprot.org/citations/9041391 | http://purl.uniprot.org/core/name | "J Clin Microbiol"xsd:string |
| http://purl.uniprot.org/citations/9041391 | http://purl.org/dc/terms/identifier | "doi:10.1128/jcm.35.3.570-577.1997"xsd:string |
| http://purl.uniprot.org/citations/9041391 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/9041391 |
| http://purl.uniprot.org/citations/9041391 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/9041391 |
| http://purl.uniprot.org/citations/9041391 | http://purl.uniprot.org/core/author | "Ando T."xsd:string |
| http://purl.uniprot.org/citations/9041391 | http://purl.uniprot.org/core/author | "Ando T."xsd:string |
| http://purl.uniprot.org/citations/9041391 | http://purl.uniprot.org/core/author | "Glass R.I."xsd:string |
| http://purl.uniprot.org/citations/9041391 | http://purl.uniprot.org/core/author | "Glass R.I."xsd:string |
| http://purl.uniprot.org/citations/9041391 | http://purl.uniprot.org/core/author | "Monroe S.S."xsd:string |
| http://purl.uniprot.org/citations/9041391 | http://purl.uniprot.org/core/author | "Monroe S.S."xsd:string |
| http://purl.uniprot.org/citations/9041391 | http://purl.uniprot.org/core/author | "Noel J.S."xsd:string |
| http://purl.uniprot.org/citations/9041391 | http://purl.uniprot.org/core/author | "Noel J.S."xsd:string |
| http://purl.uniprot.org/citations/9041391 | http://purl.uniprot.org/core/date | "1997"xsd:gYear |
| http://purl.uniprot.org/citations/9041391 | http://purl.uniprot.org/core/date | "1997"xsd:gYear |
| http://purl.uniprot.org/citations/9041391 | http://purl.uniprot.org/core/pages | "570-577"xsd:string |
| http://purl.uniprot.org/citations/9041391 | http://purl.uniprot.org/core/pages | "570-577"xsd:string |
| http://purl.uniprot.org/citations/9041391 | http://purl.uniprot.org/core/title | "A one-tube method of reverse transcription-PCR to efficiently amplify a 3-kilobase region from the RNA polymerase gene to the poly(A) tail of small round-structured viruses (Norwalk-like viruses)."xsd:string |
| http://purl.uniprot.org/citations/9041391 | http://purl.uniprot.org/core/title | "A one-tube method of reverse transcription-PCR to efficiently amplify a 3-kilobase region from the RNA polymerase gene to the poly(A) tail of small round-structured viruses (Norwalk-like viruses)."xsd:string |
| http://purl.uniprot.org/citations/9041391 | http://purl.uniprot.org/core/volume | "35"xsd:string |
| http://purl.uniprot.org/citations/9041391 | http://purl.uniprot.org/core/volume | "35"xsd:string |
| http://purl.uniprot.org/citations/9041391 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/9041391 |