| http://purl.uniprot.org/citations/9083014 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9083014 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9083014 | http://www.w3.org/2000/01/rdf-schema#comment | "The cop operon of Enterococcus hirae effects copper homeostasis in this organism. It encodes a repressor, CopY, an activator, CopZ, and two P-type copper ATPases, CopA and CopB. Expression of all four genes is regulated by the ambient copper. In this regulation, CopY apparently acts as a copper-inducible repressor. By DNase I footprinting, it was shown that purified CopY protected two discrete sites in the region encompassing nucleotides -71 to -11 relative to the translational start site and containing hyphenated inverted repeats. Transcription is initiated between these repeats at nucleotide -42, in a domain that remained accessible to DNase I in the DNA-repressor complex. Chemical cross-linking revealed that CopY exists as a dimer in solution. In DNA band-shift assays, it was apparent that the CopY-DNA interaction occurred in two discrete steps. Half-maximal binding of repressor to the two operator sites was observed at 2 x 10(-9) M and 5 x 10(-9) M CopY, respectively. Copper ions released CopY from the promoter/operator with an apparent half-binding constant for Cu(I) of 20 microM. The site-directed mutations A-61T and A-30T essentially abolished the binding of CopY to the respective binding sites, and the double mutation A-61T/A-30T inactivated both binding sites. Thus, CopY is a copper-inducible repressor of the cop operon of E. hirae, exhibiting highly specific DNA-protein interactions with two sites on the cop promoter/operator and playing a key role in copper homeostasis in E. hirae."xsd:string |
| http://purl.uniprot.org/citations/9083014 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.272.14.8932"xsd:string |
| http://purl.uniprot.org/citations/9083014 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.272.14.8932"xsd:string |
| http://purl.uniprot.org/citations/9083014 | http://purl.uniprot.org/core/author | "Solioz M."xsd:string |
| http://purl.uniprot.org/citations/9083014 | http://purl.uniprot.org/core/author | "Solioz M."xsd:string |
| http://purl.uniprot.org/citations/9083014 | http://purl.uniprot.org/core/author | "Strausak D."xsd:string |
| http://purl.uniprot.org/citations/9083014 | http://purl.uniprot.org/core/author | "Strausak D."xsd:string |
| http://purl.uniprot.org/citations/9083014 | http://purl.uniprot.org/core/date | "1997"xsd:gYear |
| http://purl.uniprot.org/citations/9083014 | http://purl.uniprot.org/core/date | "1997"xsd:gYear |
| http://purl.uniprot.org/citations/9083014 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/9083014 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/9083014 | http://purl.uniprot.org/core/pages | "8932-8936"xsd:string |
| http://purl.uniprot.org/citations/9083014 | http://purl.uniprot.org/core/pages | "8932-8936"xsd:string |
| http://purl.uniprot.org/citations/9083014 | http://purl.uniprot.org/core/title | "CopY is a copper-inducible repressor of the Enterococcus hirae copper ATPases."xsd:string |
| http://purl.uniprot.org/citations/9083014 | http://purl.uniprot.org/core/title | "CopY is a copper-inducible repressor of the Enterococcus hirae copper ATPases."xsd:string |
| http://purl.uniprot.org/citations/9083014 | http://purl.uniprot.org/core/volume | "272"xsd:string |
| http://purl.uniprot.org/citations/9083014 | http://purl.uniprot.org/core/volume | "272"xsd:string |
| http://purl.uniprot.org/citations/9083014 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/9083014 |
| http://purl.uniprot.org/citations/9083014 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/9083014 |
| http://purl.uniprot.org/citations/9083014 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/9083014 |
| http://purl.uniprot.org/citations/9083014 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/9083014 |
| http://purl.uniprot.org/uniprot/Q47839 | http://purl.uniprot.org/core/citation | http://purl.uniprot.org/citations/9083014 |
| http://purl.uniprot.org/uniprot/Q47839#attribution-B6514BF37FD0AF080BD1EAE6B6E5607B | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/9083014 |