| http://purl.uniprot.org/citations/9085271 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9085271 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9085271 | http://www.w3.org/2000/01/rdf-schema#comment | "Prophenoloxidase (pro-PO), a precursor of phenol oxidase (PO), was purified from the hemolymph of coleopteran Holotrichia diomphalia larvae. The enzyme was purified to apparent homogeneity, in six steps of chromatography, using Sephadex G-100, CM-52, Dextran-sulfate Sepharose CL-6B, Phenyl Sepharose CL-4B, Sephacryl S-200, and a Mono-Q column. The preparation exhibited a single band on SDS-PAGE. The proenzyme had a molecular weight of 158 kDa, as estimated by gel filtration. On SDS-PAGE under reducing conditions, it gave a 79 kDa band, indicating that it forms a dimer of the 79 kDa protein. On the other hand, the purified pro-PO gave two well-separated peaks, named pro-PO-1 and pro-PO-2, on reverse-phase HPLC. Amino acid compositions of both proteins were indistinguishable, thereby suggesting the presence of an allelic variant or an isoprotein. On dextran-sulfate Sepharose CL-6B chromatography, a fraction containing prophenoloxidase activating enzyme(s) (PPAE fraction), free from pro-PO, was also separated. In reconstitution experiments, the activation of purified pro-PO by PPAE fraction, was observed in the presence of 5 mM Ca2+, with a specific limited proteolysis. The NH2-terminal sequence of generated PO was determined to be NH2-Phe-Gly-Glu-Asp-Asp-. The activated PO oxidized o-diphenols but did not oxidize mono-phenol and p-diphenol substrates. The purified pro-PO was not activated by trypsin, alpha-chymotrypsin, and SDS."xsd:string |
| http://purl.uniprot.org/citations/9085271 | http://purl.uniprot.org/core/author | "Choi J.S."xsd:string |
| http://purl.uniprot.org/citations/9085271 | http://purl.uniprot.org/core/author | "Choi J.S."xsd:string |
| http://purl.uniprot.org/citations/9085271 | http://purl.uniprot.org/core/author | "Lee S.Y."xsd:string |
| http://purl.uniprot.org/citations/9085271 | http://purl.uniprot.org/core/author | "Lee S.Y."xsd:string |
| http://purl.uniprot.org/citations/9085271 | http://purl.uniprot.org/core/author | "Iwanaga S."xsd:string |
| http://purl.uniprot.org/citations/9085271 | http://purl.uniprot.org/core/author | "Iwanaga S."xsd:string |
| http://purl.uniprot.org/citations/9085271 | http://purl.uniprot.org/core/author | "Lee J.H."xsd:string |
| http://purl.uniprot.org/citations/9085271 | http://purl.uniprot.org/core/author | "Lee J.H."xsd:string |
| http://purl.uniprot.org/citations/9085271 | http://purl.uniprot.org/core/author | "Kawabata S."xsd:string |
| http://purl.uniprot.org/citations/9085271 | http://purl.uniprot.org/core/author | "Kawabata S."xsd:string |
| http://purl.uniprot.org/citations/9085271 | http://purl.uniprot.org/core/author | "Lee B.L."xsd:string |
| http://purl.uniprot.org/citations/9085271 | http://purl.uniprot.org/core/author | "Lee B.L."xsd:string |
| http://purl.uniprot.org/citations/9085271 | http://purl.uniprot.org/core/author | "Kwon T.H."xsd:string |
| http://purl.uniprot.org/citations/9085271 | http://purl.uniprot.org/core/author | "Kwon T.H."xsd:string |
| http://purl.uniprot.org/citations/9085271 | http://purl.uniprot.org/core/date | "1997"xsd:gYear |
| http://purl.uniprot.org/citations/9085271 | http://purl.uniprot.org/core/date | "1997"xsd:gYear |
| http://purl.uniprot.org/citations/9085271 | http://purl.uniprot.org/core/name | "Mol. Cells"xsd:string |
| http://purl.uniprot.org/citations/9085271 | http://purl.uniprot.org/core/name | "Mol. Cells"xsd:string |
| http://purl.uniprot.org/citations/9085271 | http://purl.uniprot.org/core/pages | "90-97"xsd:string |
| http://purl.uniprot.org/citations/9085271 | http://purl.uniprot.org/core/pages | "90-97"xsd:string |
| http://purl.uniprot.org/citations/9085271 | http://purl.uniprot.org/core/title | "Purification and characterization of prophenoloxidase from the hemolymph of coleopteran insect, Holotrichia diomphalia larvae."xsd:string |
| http://purl.uniprot.org/citations/9085271 | http://purl.uniprot.org/core/title | "Purification and characterization of prophenoloxidase from the hemolymph of coleopteran insect, Holotrichia diomphalia larvae."xsd:string |