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http://purl.uniprot.org/citations/9110174http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/9110174http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/9110174http://www.w3.org/2000/01/rdf-schema#comment"A total of 100 kb of DNA derived from 69 individual human brain cDNA clones of 0.7-2.0 kb were sequenced by concatenated cDNA sequencing (CCS), whereby multiple individual DNA fragments are sequenced simultaneously in a single shotgun library. The method yielded accurate sequences and a similar efficiency compared with other shotgun libraries constructed from single DNA fragments (> 20 kb). Computer analyses were carried out on 65 cDNA clone sequences and their corresponding end sequences to examine both nucleic acid and amino acid sequence similarities in the databases. Thirty-seven clones revealed no DNA database matches, 12 clones generated exact matches (> or = 98% identity), and 16 clones generated nonexact matches (57%-97% identity) to either known human or other species genes. Of those 28 matched clones, 8 had corresponding end sequences that failed to identify similarities. In a protein similarity search, 27 clone sequences displayed significant matches, whereas only 20 of the end sequences had matches to known protein sequences. Our data indicate that full-length cDNA insert sequences provide significantly more nucleic acid and protein sequence similarity matches than expressed sequence tags (ESTs) for database searching."xsd:string
http://purl.uniprot.org/citations/9110174http://purl.org/dc/terms/identifier"doi:10.1101/gr.7.4.353"xsd:string
http://purl.uniprot.org/citations/9110174http://purl.org/dc/terms/identifier"doi:10.1101/gr.7.4.353"xsd:string
http://purl.uniprot.org/citations/9110174http://purl.uniprot.org/core/author"Andersson B."xsd:string
http://purl.uniprot.org/citations/9110174http://purl.uniprot.org/core/author"Andersson B."xsd:string
http://purl.uniprot.org/citations/9110174http://purl.uniprot.org/core/author"Ding Y."xsd:string
http://purl.uniprot.org/citations/9110174http://purl.uniprot.org/core/author"Ding Y."xsd:string
http://purl.uniprot.org/citations/9110174http://purl.uniprot.org/core/author"Gibbs R.A."xsd:string
http://purl.uniprot.org/citations/9110174http://purl.uniprot.org/core/author"Gibbs R.A."xsd:string
http://purl.uniprot.org/citations/9110174http://purl.uniprot.org/core/author"Liu W."xsd:string
http://purl.uniprot.org/citations/9110174http://purl.uniprot.org/core/author"Liu W."xsd:string
http://purl.uniprot.org/citations/9110174http://purl.uniprot.org/core/author"Muzny D.M."xsd:string
http://purl.uniprot.org/citations/9110174http://purl.uniprot.org/core/author"Muzny D.M."xsd:string
http://purl.uniprot.org/citations/9110174http://purl.uniprot.org/core/author"Yu W."xsd:string
http://purl.uniprot.org/citations/9110174http://purl.uniprot.org/core/author"Yu W."xsd:string
http://purl.uniprot.org/citations/9110174http://purl.uniprot.org/core/author"Worley K.C."xsd:string
http://purl.uniprot.org/citations/9110174http://purl.uniprot.org/core/author"Worley K.C."xsd:string
http://purl.uniprot.org/citations/9110174http://purl.uniprot.org/core/author"Lennon G."xsd:string
http://purl.uniprot.org/citations/9110174http://purl.uniprot.org/core/author"Lennon G."xsd:string
http://purl.uniprot.org/citations/9110174http://purl.uniprot.org/core/author"Ricafrente J.Y."xsd:string
http://purl.uniprot.org/citations/9110174http://purl.uniprot.org/core/author"Ricafrente J.Y."xsd:string
http://purl.uniprot.org/citations/9110174http://purl.uniprot.org/core/author"Wentland M.A."xsd:string
http://purl.uniprot.org/citations/9110174http://purl.uniprot.org/core/author"Wentland M.A."xsd:string