| http://purl.uniprot.org/citations/9116495 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9116495 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9116495 | http://www.w3.org/2000/01/rdf-schema#comment | "ATP:citrate lyase (ACL) is a major generator of cytosolic acetyl-coenzymeA, which is required for both fatty acid and cholesterol biosynthesis. The human ACL (hACL) cDNA was cloned by RT-PCR, and our results indicate the existence of previously unknown sequence variations in hACL. Expression of the hACL cDNA in Spodoptera frugiperda 9 insect cells resulted in the production of high levels of soluble, active enzyme. The recombinant protein (re-hACL) was purified to homogeneity from the soluble lysate of infected cells and was observed to exist as a tetramer by gel filtration chromatography. Kinetic analyses indicated that the re-hACL and rat ACL have very similar enzymological properties. The facile preparation of milligram quantities of purified, active re-hACL affords the opportunity to characterize the enzyme for structure-based design of hypolipidemic drugs, and to further examine the functional significance of the sequence variations."xsd:string |
| http://purl.uniprot.org/citations/9116495 | http://purl.org/dc/terms/identifier | "doi:10.1006/prep.1996.0668"xsd:string |
| http://purl.uniprot.org/citations/9116495 | http://purl.org/dc/terms/identifier | "doi:10.1006/prep.1996.0668"xsd:string |
| http://purl.uniprot.org/citations/9116495 | http://purl.uniprot.org/core/author | "Wang X.M."xsd:string |
| http://purl.uniprot.org/citations/9116495 | http://purl.uniprot.org/core/author | "Wang X.M."xsd:string |
| http://purl.uniprot.org/citations/9116495 | http://purl.uniprot.org/core/author | "Bentley R."xsd:string |
| http://purl.uniprot.org/citations/9116495 | http://purl.uniprot.org/core/author | "Bentley R."xsd:string |
| http://purl.uniprot.org/citations/9116495 | http://purl.uniprot.org/core/author | "Bruckner R.C."xsd:string |
| http://purl.uniprot.org/citations/9116495 | http://purl.uniprot.org/core/author | "Bruckner R.C."xsd:string |
| http://purl.uniprot.org/citations/9116495 | http://purl.uniprot.org/core/author | "Chadwick C.C."xsd:string |
| http://purl.uniprot.org/citations/9116495 | http://purl.uniprot.org/core/author | "Chadwick C.C."xsd:string |
| http://purl.uniprot.org/citations/9116495 | http://purl.uniprot.org/core/author | "Ciccarelli R.B."xsd:string |
| http://purl.uniprot.org/citations/9116495 | http://purl.uniprot.org/core/author | "Ciccarelli R.B."xsd:string |
| http://purl.uniprot.org/citations/9116495 | http://purl.uniprot.org/core/author | "Lord K.A."xsd:string |
| http://purl.uniprot.org/citations/9116495 | http://purl.uniprot.org/core/author | "Lord K.A."xsd:string |
| http://purl.uniprot.org/citations/9116495 | http://purl.uniprot.org/core/author | "Loscig J."xsd:string |
| http://purl.uniprot.org/citations/9116495 | http://purl.uniprot.org/core/author | "Loscig J."xsd:string |
| http://purl.uniprot.org/citations/9116495 | http://purl.uniprot.org/core/author | "O'Connor B."xsd:string |
| http://purl.uniprot.org/citations/9116495 | http://purl.uniprot.org/core/author | "O'Connor B."xsd:string |
| http://purl.uniprot.org/citations/9116495 | http://purl.uniprot.org/core/author | "Simmons S.J."xsd:string |
| http://purl.uniprot.org/citations/9116495 | http://purl.uniprot.org/core/author | "Simmons S.J."xsd:string |
| http://purl.uniprot.org/citations/9116495 | http://purl.uniprot.org/core/author | "Smallwood A."xsd:string |
| http://purl.uniprot.org/citations/9116495 | http://purl.uniprot.org/core/author | "Smallwood A."xsd:string |